2026-05-30T01:10:57Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/311382026-02-03T11:24:30Zcom_10630_2254col_10630_37953

The Mangotoxin Biosynthetic Operon (mbo) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution. Carrión Bravo, Víctor José Gutiérrez Barranquero, José Antonio Arrebola-Díez, Eva María Bardaji, Leire Codina, Juan C. De-Vicente-Moreno, Antonio Cazorla-López, Francisco Manuel Murillo, Jesús Fitotoxinas Antimetabolitos Bacterias fitopatógenas Operones Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III; however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex. 2024-04-24T07:07:32Z 2024-04-24T07:07:32Z 2024-04-24T07:07:32Z 2013-01-14 journal article Carrión VJ, Gutiérrez-Barranquero JA, Arrebola E, Bardaji L, Codina JC, de Vicente A, Cazorla FM, Murillo J. The mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolution. Appl Environ Microbiol. 2013 Feb;79(3):756-67. https://hdl.handle.net/10630/31138 10.1128/AEM.03007-12 eng open access ASM Journals