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A Proteomic Study of the Bioactivity of Annona muricata Leaf Extracts in HT-1080 Fibrosarcoma Cells. Marrero Capitán, Ana Dácil Rodríguez-Quesada, Ana María Martínez-Póveda, Beatriz Amparo Medina-Torres, Miguel Ángel Cárdenas, Casimiro Cáncer - Investigación Frutas tropicales - Uso terapéutico - Ensayos Anonáceas - Uso terapéutico - Ensayos Ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) Annona muricata Graviola Soursop Cancer Cell cycle Iron metabolism Ferroptosis Graviola (Annona muricata) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic approach to detect altered patterns in proteins on both conditioned media and extracts of HT-1080 fibrosarcoma cells under treatment conditions, revealing new potential bioactivities of Annona muricata extracts. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from Annona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated in vitro. Additional experimental data reveal that DMSO extracts protect HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells from ferroptosis. Data from our proteomic study are available via ProteomeXchange with identifier PXD042354. Partial funding for open access charge: Universidad de Málaga 2024-06-18T11:39:04Z 2024-06-18T11:39:04Z 2024-06-18 2023-07-27 journal article VoR Marrero, A. D., Quesada, A. R., Martínez-Poveda, B., Medina, M. Á., & Cárdenas, C. (2023). A Proteomic Study of the Bioactivity of Annona muricata Leaf Extracts in HT-1080 Fibrosarcoma Cells. International Journal Of Molecular Sciences, 24(15), 12021. https://doi.org/10.3390/ijms241512021 https://hdl.handle.net/10630/31652 10.3390/ijms241512021 eng Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ open access application/pdf MDPI