2026-06-05T15:41:42Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/323792026-02-03T11:19:26Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Gentile, Adriana Mariel
author
Lhamyani, Said
author
Coín-Aragüez, Leticia I.
author
Clemente-Postigo, María Mercedes
author
Oliva Olivera, Wilfredo
author
Romero-Zerbo, Silvana Yanina
author
García Serrano, Sara
author
García-Escobar, Eva
author
Zayed, Hatem
author
Doblado, Esther
author
Bermúdez Silva, Francisco Javier
author
Murri, Mora
author
Tinahones-Madueño, Francisco José
author
El-Bekay, Rajaa
2024-07-31T09:38:32Z
2024-07-31T09:38:32Z
2019
Gentile, A.-M., Lhamyani, S., Coín-Aragüez, L., Clemente-Postigo, M., Oliva Olivera, W., Romero-Zerbo, S.-Y., García-Serrano, S., García-Escobar, E., Zayed, H., Doblado, E., Bermúdez-Silva, F.-J., Murri, M., Tinahones, F.J. and El Bekay, R. (2019), miR-20b, miR-296, and Let-7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes. Obesity, 27: 245-254. https://doi.org/10.1002/oby.22363
https://hdl.handle.net/10630/32379
10.1002/oby.22363
Objective: This study aimed to analyze the potential association of different microRNA (miRNA) moleculeswith both type 2 diabetes (T2D) and obesity and determine their target genes.Methods: Quantitative PCR was used to analyze the miR-20b, miR-296, and Let-7f levels in human visceraland subcutaneous adipose tissues (ATs) in relation to obesity and T2D, miRTarBase 4.0 was used for valida-tion of target genes, and the Protein Analysis Through Evolutionary Relationships (PANTHER) ClassificationSystem and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to an-notate the biological processes of the predicted targets.Results: In AT, miR-20b, miR-296, and Let-7f levels were significantly different between normoglycemicsubjects and those with T2D. In visceral adipose tissue, miRNA levels were higher in normoglycemic/obesitysamples than in T2D/obesity samples. miR-20b, miR-296, and Let-7f target genes that showed significantdifferences in both ATs in relation to obesity and T2D were CDKN1A, CX3CL1, HIF1A, PPP2R1B, STAT3, andVEGFA. These genes are known to be principally involved in the vascular endothelial growth factor (VEGF)and WNT pathways.Conclusions: This study provides experimental evidence of the possible correlation between AT miR-20b,miR-296, and Let-7f with obesity and T2D, which might involve vascular endothelial growth factor and WNT-dependent pathways that are regulated by six different genes, suggesting a novel signaling pathway thatcould be important for understanding the mechanisms underlying the AT dysfunction associated with obe-sity and T2D
eng
Atribución 4.0 Internacional
Obesidad - Investigación
Diabetes - Investigación
miR-20b, miR-296, and Let-7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes.
journal article
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