2026-06-02T17:39:05Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/324642026-02-03T10:48:40Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Yu, Gang author Derkacheva, Maria author Rufián Plaza, José Sebastián author Brillada, Carla author Kowarschik, Kathrin author Jiang, Shushu author Debryshire, Paul author Ma, Miaomiao author DeFalco, Thomas A author Morcillo, Rafael J author Stransfeld, Lena author Wei, Yali author Zhou, Jian-Min author Menke, Frank L author Trujillo, Marco author Zipfel, Cyril author Macho, Alberto P author 2022 Plant immunity is tightly controlled by a complex and dynamic regulatory network, which ensures optimal activation upon detection of potential pathogens. Accordingly, each component of this network is a potential target for manipulation by pathogens. Here, we report that RipAC, a type III‐secreted effector from the bacterial pathogen Ralstonia solanacearum, targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern‐triggered immunity (PTI). PUB4 plays a positive role in PTI by regulating the homeostasis of the central immune kinase BIK1. Before PAMP perception, PUB4 promotes the degradation of non‐activated BIK1, while after PAMP perception, PUB4 contributes to the accumulation of activated BIK1. RipAC leads to BIK1 degradation, which correlates with its PTI‐inhibitory activity. RipAC causes a reduction in pathogen‐associated molecular pattern (PAMP)‐induced PUB4 accumulation and phosphorylation. Our results shed light on the role played by PUB4 in immune regulation, and illustrate an indirect targeting of the immune signalling hub BIK1 by a bacterial effector. Yu G, Derkacheva M, Rufian JS, Brillada C, Kowarschik K, Jiang S, Derbyshire P, Ma M, DeFalco TA, Morcillo RJL, Stransfeld L, Wei Y, Zhou JM, Menke FLH, Trujillo M, Zipfel C, Macho AP. The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type-III effector. EMBO J. 2022 Dec 1;41(23):e107257. doi: 10.15252/embj.2020107257. Epub 2022 Oct 31 https://hdl.handle.net/10630/32464 10.15252/embj.2020107257 Inmunología vegetal The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 and is targeted by a bacterial type‐III effector