2026-06-02T11:57:27Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/325552026-02-03T11:34:03Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc García-Márquez, Jorge author Galafat Díaz, Alba author Molina-Roque, Luis author Domínguez-Maqueda, Marta author De las Heras, Verónica author Simó-Mirabet, Paula author Vizcaíno, Antonio J. author Martos-Sitcha, Juan Antonio author Alarcón López, Francisco Javier author Moriñigo-Gutiérrez, Miguel Ángel author Balebona-Accino, María del Carmen author 2024-09-11 This study investigates the postbiotic potential of extracellular products (ECPs) from Bacillus pumilus strains cultivated on microalgae-supplemented media. We assessed enzymatic and antimicrobial activities to select ECPs that enhance the digestive processes in gilthead seabream. Additionally, we explored the in vitro enzymatic capacity of the chosen postbiotics to hydrolyze macromolecules in microalgae. Finally, a feeding trial was conducted to determine the in vivo effects of the ECPs on Sparus aurata. In vitro enzymatic assays demonstrated diverse hydrolytic capacities among ECPs. All conditions exhibited antimicrobial activity against Photobacterium damselae subsp. piscicida, with variation in inhibitory effects against Vibrio harveyi and Tenacibaculum maritimum. Furthermore, in vitro assays revealed differences in protein hydrolysis and soluble protein concentration, influencing amino acid and reducing sugar release from microalgal biomass. These analyses facilitated a selection to test ECPs in vivo. Lastly, the in vivo experiment revealed no differences in the growth performance, nutrient utilization, and general metabolism of S. aurata fed the experimental diets. Dietary inclusion of postbiotics increased the activity of key digestive enzymes in fish compared to the control group, and particularly, values increased significantly when the fish were fed with the ECP-nanoparticulate-supplemented diet. In conclusion, the inclusion of microalgae in the culture media significantly influences the activity of extracellular products from B. pumilus strains, as evidenced in both in vitro and in vivo assays. García-Márquez, J., Díaz, A.G., Molina-Roque, L. et al. Microalgal and Cyanobacterial Biomasses Modified the Activity of Extracellular Products from Bacillus pumilus: An In Vitro and In Vivo Assessment. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10350-z https://hdl.handle.net/10630/32555 10.1007/s12602-024-10350-z Acuicultura Microalgas Microalgal and Cyanobacterial Biomasses Modified the Activity of Extracellular Products from Bacillus pumilus: An In Vitro and In Vivo Assessment