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oai:riuma.uma.es:10630/326232026-02-03T12:06:46Zcom_10630_2254col_10630_37959

TRIM5-mediated retrovirus restriction is modulated by type I interferon. Ortega-Prieto, Ana María Boehmer, Nina Dinc, Hanife Betancor, Gilberto Petrosyan, Eduard Malim, Michael H. Jiménez-Guardeño, José Manuel Virología Interferón Virology HIV TRIM5 Virus Since the identification in 2004 of the interferon-stimulated gene (ISG) tripartite motif-containing protein 5 α (TRIM5α) from rhesus macaques as a restriction factor preventing HIV-1 infection in these monkeys, the antiretroviral activity of several primate TRIM5α orthologs against HIV-1 has been described, establishing the model that TRIM5α inhibits retroviral infection in a species-specific manner, preventing host cell infection by retroviruses from different species through fragmentation of incoming viral capsids and the activation of innate immune pathways. However, the long held dogma that retroviruses have evolved to evade the TRIM5α ortholog present in species to which they are endemic has recently changed by the identification of human TRIM5α as a major determinant in the Type 1 IFNinduced suppression of HIV-1 replication, presumably contributing to the immune control of HIV-1 in infected humans. Given that IFN levels are elevated during natural retrovirus infection and that IFN treatment enables human TRIM5α restriction of HIV-1, we evaluated the IFN-induced restriction of distinct retroviruses in presence of TRIM5α orthologues from different primate species. To this end, we ectopically expressed different TRIM5α orthologues in human U87 cells where endogenous TRIM5α and MX2 expression had been ablated using CRISPR–Cas9 genome editing, and then challenged with a wide range of GFP-encoding retrovirus-based vectors in the presence or absence of IFN. This approach reveals that IFN treatment changes the patterns of TRIM5α-mediated retrovirus restriction, suggesting that the role of TRIM5α in retrovirus infection should be re-examined under conditions that more closely mimic those encountered during natural virus infection. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. 2024-09-18T11:34:33Z 2024-09-18T11:34:33Z 2024 conference output https://hdl.handle.net/10630/32623 eng XVII Congreso Nacional de Virología Santiago de Compostela, España Septiembre 2024 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ open access application/pdf