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oai:riuma.uma.es:10630/327092026-02-03T11:11:10Zcom_10630_2254col_10630_37953

Induction of superficial zone protein (SZP)/ lubricin/PRG 4 in muscle-derived mesenchymal stem/progenitor cells by transforming growth factor-b1 and bone morphogenetic protein-7 Andrades-Gómez, José Antonio Motaung, Shirley C Jiménez-Palomo, Pedro Claros-Gil, Silvia López-Puerta, José M. Becerra-Ratia, José Schmid, Thomas M. Reddi, A. Hari Cartílagos Introduction Articular cartilage (AC) is an avascular tissue with precise polarity and organization. The three distinct zones are: surface, middle and deep. The production and accumulation of the superficial zone protein (SZP), also known as lubricin, by the surface zone is a characteristic feature of AC. To date, there is a wealth of evidence showing differentiation of AC from mesenchymal stem cells. Most studies that described chondrogenic differentiation did not focus on AC with characteristic surface marker SZP/lubricin. The present investigation was initiated to determine the induction of SZP/lubricin in skeletal muscle-derived mesenchymal stem/progenitor cells (MDMSCs) by transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-7 (BMP-7). Methods MDMSCs were cultured as a monolayer at a density of 1 × 105 cells/well in 12-well tissue culture plates. Cell cultures were treated for 3, 7 and 10 days with TGF-β1 and BMP-7. The medium was analyzed for SZP. The cells were used to isolate RNA for RT-PCR assays for SZP expression. Results The SZP/lubricin increased in a time-dependent manner on Days 3, 7 and 10 in the medium. As early as Day 3, there was a three-fold increase in response to 3 ng/ml of TGF-β1 and 300 ng/ml of BMP-7. This was confirmed by immunochemical localization of SZP as early as Day 3 after treatment with TGF-β1. The expression of SZP mRNA was enhanced by TGF-β1. Conclusions The present investigation demonstrated the efficient and reproducible induction of SZP/lubricin accumulation by TGF-β1 and BMP-7 in skeletal MDMSCs. Optimization of the experimental conditions may permit the utility of MDMSCs in generating surface zone-like cells with phenotypic markers of AC and, therefore, constitute a promising cell source for tissue engineering approaches of superficial zone cartilage. 2024-09-20T08:58:44Z 2024-09-20T08:58:44Z 2024-09-20T08:58:44Z 2012-04-10 journal article Andrades, J.A., Motaung, S.C., Jiménez-Palomo, P. et al. Induction of superficial zone protein (SZP)/lubricin/PRG 4 in muscle-derived mesenchymal stem/progenitor cells by transforming growth factor-β1 and bone morphogenetic protein-7. Arthritis Res Ther 14, R72 (2012). https://doi.org/10.1186/ar3793 https://hdl.handle.net/10630/32709 10.1186/ar3793 eng open access Springer Nature