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oai:riuma.uma.es:10630/330012026-02-03T11:03:33Zcom_10630_2254col_10630_37953

Molina-Santiago, Carlos Pearson, John Navarro, Yurena Berlanga-Clavero, María Victoria Caraballo-Rodríguez, Andrés Mauricio Petras, Daniel García Martín, Maria Luisa Lamon, Gaelle Habenstein, Birgit Cazorla-López, Francisco Manuel De-Vicente-Moreno, Antonio Loquet, Antoine Dorrestein, Pieter Romero-Hinojosa, Diego Francisco 2024-09-24T09:56:02Z 2024-09-24T09:56:02Z 2019-04-23 Molina-Santiago, C., Pearson, J.R., Navarro, Y. et al. The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization. Nat Commun 10, 1919 (2019). https://doi.org/10.1038/s41467-019-09944-x https://hdl.handle.net/10630/33001 10.1038/s41467-019-09944-x Bacteria of the genera Pseudomonas and Bacillus can promote plant growth and protect plants from pathogens. However, the interactions between these plant-beneficial bacteria are understudied. Here, we explore the interaction between Bacillus subtilis 3610 and Pseudomonas chlororaphis PCL1606. We show that the extracellular matrix protects B. subtilis colonies from infiltration by P. chlororaphis. The absence of extracellular matrix results in increased fluidity and loss of structure of the B. subtilis colony. The P. chlororaphis type VI secretion system (T6SS) is activated upon contact with B. subtilis cells, and stimulates B. subtilis sporulation. Furthermore, we find that B. subtilis sporulation observed prior to direct contact with P. chlororaphis is mediated by histidine kinases KinA and KinB. Finally, we demonstrate the importance of the extracellular matrix and the T6SS in modulating the coexistence of the two species on melon plant leaves and seeds. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 Internacional Biopelículas Relaciones planta-microbio The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization. journal article