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oai:riuma.uma.es:10630/332032026-02-03T10:55:03Zcom_10630_2254col_10630_37953

Rodriguez-de-Fonseca, Fernando Medina-Paz, Francisco Sapozhnikov, Mira Hurtado-Guerrero, Isaac Rubio-Lamia, Leticia Olga Martín-de-las-Heras, Stella Requena-Ocaña, Nerea Flores-López, María Fernández-Arjona, María del Mar Rivera-González, Patricia Serrano, Antonia Serrano-Castro, Pedro Jesús Zapico, Sara C. Suárez-Pérez, Juan 2024-09-25T10:13:39Z 2024-09-25T10:13:39Z 2024 Rodríguez de Fonseca, F.; Medina-Paz, F.; Sapozhnikov, M.; Hurtado-Guerrero, I.; Rubio, L.; Martín-de-las-Heras, S.; RequenaOcaña, N.; Flores-López, M.; Fernández-Arjona, M.d.M.; Rivera, P.; et al. Plasma Concentrations of High Mobility Group Box 1 Proteins and Soluble Receptors for Advanced Glycation End-Products Are Relevant Biomarkers of Cognitive Impairment in Alcohol Use Disorder: A Pilot Study. Toxics 2024, 12, 190. https://doi.org/10.3390/toxics12030190 https://hdl.handle.net/10630/33203 https://doi.org/10.3390/toxics12030190 Alcohol use disorder (AUD) is a major component in the etiology of cognitive decline and dementia. Underlying mechanisms by which long-term alcohol abuse causes cognitive dysfunction include excessive oxidative stress and inflammation in the brain, activated by increased reactive oxygen/nitrogen species (ROS/RNS), advanced glycation end-products (AGEs) and high-mobility group box 1 protein (HMGB1). In a pilot study, we examine the potential clinical value of circulating biomarkers of oxidative stress including ROS/RNS, HMGB1, the soluble receptor for AGE (sRAGE), the brain biomarker of aging apolipoprotein D (ApoD), and the antioxidant regulator nuclear factor erythroid 2-related factor 2 (NRF2) as predictive indices for cognitive impairment (CI) in abstinent patients with AUD (n = 25) compared to patients with established Alzheimer's disease (AD, n = 26) and control subjects (n = 25). Plasma concentrations of sRAGE were evaluated with immunoblotting; ROS/RNS with a fluorometric kit; and HMGB1, ApoD, and NRF2 by ELISA. Abstinent AUD patients had higher sRAGE, ROS/RNS (p < 0.05), and ApoD (p < 0.01) concentrations, similar to those of AD patients, and lower NRF2 (p < 0.01) concentrations, compared to controls. These changes were remarkable in AUD patients with CI. HMGB1, and sRAGE correlated positively with duration of alcohol use (rho = 0.398, p = 0.022; rho = 0.404, p = 0.018), whereas sRAGE correlated negatively with periods of alcohol abstinence (rho = -0.340, p = 0.045). A predictive model including ROS/RNS, HMGB1, sRAGE, alcohol use duration, and alcohol abstinence periods was able to differentiate AUD patients with CI (92.3% of correct predictions, ROC-AUC= 0.90) from those without CI. In conclusion, we propose ROS/RNS, HMGB1, and sRAGE as stress biomarkers capable of predicting cognitive impairment in AUD patients. eng open access Plasma sanguíneo Alcohol - Consumo Plasma Concentrations of High Mobility Group Box 1 Proteins and Soluble Receptors for Advanced Glycation End-Products Are Relevant Biomarkers of Cognitive Impairment in Alcohol Use Disorder: A Pilot Study. journal article