2026-05-30T22:06:15Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/334012026-02-03T11:05:41Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Lipinski, Michal author Muñoz-Viana, Rafael author del Blanco, Beatriz author Márquez-Galera, Ángel author Medrano-Relinque, Juan author Caramés-Tejedor, José María author Szczepankiewicz, Andrzej A. author Fernández-Albert, Jordi author Navarrón, Carmen M. author Olivares, Román author Wilczyński, Grzegorz M. author Canals-Gamoneda, Santiago author López-Atalaya, José P. author Barco, Ángel author 2020-05-22 The lysine acetyltransferases type 3 (KAT3) family members CBP and p300 are important transcriptional co-activators, but their specific functions in adult post-mitotic neurons remain unclear. Here, we show that the combined elimination of both proteins in forebrain excitatory neurons of adult mice resulted in a rapidly progressing neurological phenotype associated with severe ataxia, dendritic retraction and reduced electrical activity. At the molecular level, we observed the downregulation of neuronal genes, as well as decreased H3K27 acetylation and pro-neural transcription factor binding at the promoters and enhancers of canonical neuronal genes. The combined deletion of CBP and p300 in hippocampal neurons resulted in the rapid loss of neuronal molecular identity without de- or transdifferentiation. Restoring CBP expression or lysine acetylation rescued neuronal-specific transcription in cultured neurons. Together, these experiments show that KAT3 proteins maintain the excitatory neuron identity through the regulation of histone acetylation at cell type-specific promoter and enhancer regions. Lipinski, M., Muñoz-Viana, R., del Blanco, B. et al. KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Nat Commun 11, 2588 (2020). https://hdl.handle.net/10630/33401 10.1038/s41467-020-16246-0 Ratones - Cerebro Proteínas Neurociencias KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.