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oai:riuma.uma.es:10630/335612026-02-03T11:30:54Zcom_10630_2254col_10630_37953

Claros-Gil, Silvia Rodríguez-Losada, Noela Cruz, Encarnación Guerado-Parra, Enrique Becerra-Ratia, José Andrades-Gómez, José Antonio 2024-09-27T07:00:37Z 2024-09-27T07:00:37Z 2012-09-01 Claros S, Rodríguez-Losada N, Cruz E, Guerado E, Becerra J, Andrades JA. Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System. Cell Transplantation. 2012;21(9):2021-2032. https://hdl.handle.net/10630/33561 10.3727/096368912X636939 Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and nonadherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4, and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent-, hematopoietic-, mesenchymal-, and endothelial-specific markers in vitro and can undergo osteogenic differentiation in vivo. eng open access Células madre hematopoyéticas Huesos - Crecimiento Characterization of adult stem/progenitor cell populations from bone marrow in a three-dimensional collagen gel culture system. journal article