2026-06-07T05:13:55Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/336552026-02-03T10:52:06Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Ruiz-Villalba, Adrián
author
Mattiotti, Andrea
author
Gunst, Quinn D
author
Cano-Ballesteros, Sara
author
Van, Maurice JB
author
Ruijter, JM
2024-09-27T10:07:16Z
2024-09-27T10:07:16Z
2017
Ruiz-Villalba, A., Mattiotti, A., Gunst, Q.D. et al. Reference genes for gene expression studies in the mouse heart. Sci Rep 7, 24 (2017). https://doi.org/10.1038/s41598-017-00043-9
https://hdl.handle.net/10630/33655
10.1038/s41598-017-00043-9
To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research.
eng
Corazón - Enfermedades
Reference genes for gene expression studies in the mouse heart
journal article
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Ruiz-Villalba et al, 2017.pdf