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00925njm 22002777a 4500 dc Carmona-Mejías, Rita María author Cano, E author Grueso, E author Ruiz-Villalba, A author Bera, TK author Gaztambide, J author Segovia, JC author Muñoz-Chápuli, R author 2011-05 The peritoneal mesothelium exhibits a high regenerative ability. Peritoneal regeneration is concomitant with the appearance, in the coelomic cavity, of a free-floating population of cells whose origin and functions are still under discussion. We have isolated and characterized this cell population and we have studied the process of mesothelial regeneration through flow cytometry and confocal microscopy in a murine model lethally irradiated and reconstituted with GFP-expressing bone marrow cells. In unoperated control mice, most free cells positive for mesothelin, a mesothelial marker, are green fluorescent protein (GFP). However, 24 hrs after peritoneal damage, free mesothelin+/ GFP+ cells appear in peritoneal lavages. Cultured lavage peritoneal cells show colocalization of GFP with mesothelial (mesothelin, cytokeratin) and fibroblastic markers. Immunohistochemical staining of the peritoneal wall also revealed colocalization of GFP with mesothelial markers and with procollagen-1 and smooth muscle α-actin. This was observed in the injured area as well as in the surrounding not-injured peritoneal surfaces. These cells, which we herein call peritoneal repairing cells (PRC), are very abundant 1 week after surgery covering both the damaged peritoneal wall and the surrounding uninjured area. However, they become very scarce 1 month later, when the mesothelium has completely healed. We suggest that PRC constitute a type of monocyte-derived cells, closely related with the tissue-repairing cells known as ‘fibrocytes’ and specifically involved in peritoneal reparation. Thus, our results constitute a synthesis of the different scenarios hitherto proposed about peritoneal regeneration, particularly recruitment of circulating progenitor cells and adhesion of free-floating coelomic cells. Carmona, R., Cano, E., Grueso, E., Ruiz-Villalba, A., Bera, T.K., Gaztambide, J., Segovia, J.C. and Muñoz-Chápuli, R. (2011), Peritoneal repairing cells: a type of bone marrow derived progenitor cells involved in mesothelial regeneration. Journal of Cellular and Molecular Medicine, 15: 1200-1209. https://doi.org/10.1111/j.1582-4934.2010.01087.x https://hdl.handle.net/10630/34011 10.1111/j.1582-4934.2010.01087.x Peritoneo - Enfermedades Peritoneal repairing cells: a type of bone marrow derived progenitor cells involved in mesothelial regeneration