2026-06-01T13:38:43Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/340182026-02-03T11:13:55Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Carmona-Mejías, Rita María author Cano, E author Mattiotti, A author Gaztambide, J author Muñoz-Chápuli, R author 2013-02 Gut mesodermal tissues originate from the splanchnopleural mesenchyme. However, the embryonic gastrointestinal coelomic epithelium gives rise to mesenchymal cells, whose significance and fate are little known. Our aim was to investigate the contribution of coelomic epithelium-derived cells to the intestinal development. We have used the transgenic mouse model mWt1/IRES/GFP-Cre (Wt1cre) crossed with the Rosa26R-EYFP reporter mouse. In the gastrointestinal duct Wt1, the Wilms’ tumor suppressor gene, is specific and dynamically expressed in the coelomic epithelium. In the embryos obtained from the crossbreeding, the Wt1-expressing cell lineage produces the yellow fluorescent protein (YFP) allowing for colocalization with differentiation markers through confocal microscopy and flow cytometry. Wt1cre-YFP cells were very abundant throughout the intestine during midgestation, declining in neonates. Wt1cre-YFP cells were also transiently observed within the mucosa, being apparently released into the intestinal lumen. YFP was detected in cells contributing to intestinal vascularization (endothelium, pericytes and smooth muscle), visceral musculature (circular, longitudinal and submucosal) as well as in Cajal and Cajal-like interstitial cells. Wt1cre-YFP mesenchymal cells expressed FGF9, a critical growth factor for intestinal development, as well as PDGFRα, mainly within developing villi. Thus, a cell population derived from the coelomic epithelium incorporates to the gut mesenchyme and contribute to a variety of intestinal tissues, probably playing also a signaling role. Our results support the origin of interstitial cells of Cajal and visceral circular muscle from a common progenitor expressing anoctamin-1 and SMCα-actin. Coelomic-derived cells contribute to the differentiation of at least a part of the interstitial cells of Cajal. Carmona R, Cano E, Mattiotti A, Gaztambide J, Muñoz-Chápuli R (2013) Cells Derived from the Coelomic Epithelium Contribute to Multiple Gastrointestinal Tissues in Mouse Embryos. PLoS ONE 8(2): e55890. https://doi.org/10.1371/journal.pone.0055890 https://hdl.handle.net/10630/34018 10.1371/journal.pone.0055890 Epitelio Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos