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Labella Vera, Alejandro Manuel Manchado, Manuel Alonso, María del Carmen Castro, Dolores Romalde, Jesús Borrego, Juan José 2024-09-30T11:07:26Z 2024-09-30T11:07:26Z 2010-05-07 Labella, A., Manchado, M., Alonso, M.C., Castro, D., Romalde, J.L. and Borrego, J.J. (2010), Molecular intraspecific characterization of Photobacterium damselae ssp. damselae strains affecting cultured marine fish. Journal of Applied Microbiology, 108: 2122-2132. https://doi.org/10.1111/j.1365-2672.2009.04614.x https://hdl.handle.net/10630/34026 doi:10.1111/j.1365-2672.2009.04614.x In this study, the techniques tested are confirmed as good tools for molecular typing, because they allow discrimination between P. damselae ssp. damselae strains isolated within the same outbreak. In addition, ERIC-PCR and REP-PCR methods were more adequate for rapid typing of P. damselae ssp. damselae than RAPD, allowing the discrimination at strain level. Significance and Impact of the Study: The results, in agreement with previous studies, confirmed the high intraspecific variability among isolated P. damselae ssp. damselae strains at both phenotypic and genetic levels. This suggests the existence of different clonal lineages that coexist in the same geographic area, within a short period of time (2–3 years). The discrimination at strain level can be useful to study the traceability of infections. eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Peces - Enfermedades Peces - Genética molecular Molecular intraspecific characterization of Photobacterium damselae ssp. damselae strains affecting cultured marine fish journal article