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oai:riuma.uma.es:10630/341002026-02-03T11:17:28Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Real-Domínguez, María Macarena author Molina-Molina, José-Manuel author Jimenez, Jesús author Diéguez, Horacio R. author Fernández, Mariana author Olea, Nicolás author 2015 Medicinal plants are widely used for the treatment of diseases and for the development of new drugs. This study was designed to determine the presence of hormone-like activities dependent on the activation of human estrogen receptor alpha (hERa) and/or androgen receptor (hAR) in methanol extracts prepared from three medicinal plants historically and currently used for therapeutic purposes: Ginkgo biloba leaves (GBL), Elettaria cardamomum seeds (ECS) and Plantago ovata seeds (POS). After a solid-liquid extraction (SLE) step, their effects on hERa function were assessed in MCF-7 breast cancer cells using the E-Screen bioassay, and their ability to induce hAR-mediated reporter gene expression was evaluated using the androgen-sensitive stable prostatic PALM cell line. Unlike POS extracts, GBL and ECS extracts showed estrogenic (0.07 and 0.20 nM E2Eq mg(-1), respectively) and anti-estrogenic (0.01 and 0.02 μM ICI182780Eq mg(-1), respectively) activities. ECS extracts evidenced androgenic activity (0.30 nM R1881Eq mg(-1)) and POS extracts anti-androgenic activity (22.30 μM ProcEq mg(-1)). According to these findings, these plant extracts may interfere with the endocrine system via one or more hormonal receptors, and further investigation is warranted into their role as endocrine disrupters in humans Real M, Molina-Molina JM, Jimenez J, Diéguez HR, Fernández MF, Olea N. Assessment of hormone-like activities in Ginkgo biloba, Elettaria cardamomum and Plantago ovata extracts using in vitro receptor-specific bioassays. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(9):1531-41. doi: 10.1080/19440049.2015.1071922. Epub 2015 Aug 4. PMID: 26161806. https://hdl.handle.net/10630/34100 10.1080/19440049.2015.1071922 Plantas medicinales Andrógenos Ensayos biológicos Assessment of hormone-like activities in Ginkgo biloba, Elettaria cardamomum and Plantago ovata extracts using in vitro receptor-specific bioassays