2026-05-29T23:28:19Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/341002026-02-03T11:17:28Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Real-Domínguez, María Macarena
author
Molina-Molina, José-Manuel
author
Jimenez, Jesús
author
Diéguez, Horacio R.
author
Fernández, Mariana
author
Olea, Nicolás
2024-10-01T07:19:30Z
2024-10-01T07:19:30Z
2015
Real M, Molina-Molina JM, Jimenez J, Diéguez HR, Fernández MF, Olea N. Assessment of hormone-like activities in Ginkgo biloba, Elettaria cardamomum and Plantago ovata extracts using in vitro receptor-specific bioassays. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(9):1531-41. doi: 10.1080/19440049.2015.1071922. Epub 2015 Aug 4. PMID: 26161806.
https://hdl.handle.net/10630/34100
10.1080/19440049.2015.1071922
Medicinal plants are widely used for the treatment of diseases and for the development of new drugs. This study was designed to determine the presence of hormone-like activities dependent on the activation of human estrogen receptor alpha (hERa) and/or androgen receptor (hAR) in methanol extracts prepared from three medicinal plants historically and currently used for therapeutic purposes: Ginkgo biloba leaves (GBL), Elettaria cardamomum seeds (ECS) and Plantago ovata seeds (POS). After a solid-liquid extraction (SLE) step, their effects on hERa function were assessed in MCF-7 breast cancer cells using the E-Screen bioassay, and their ability to induce hAR-mediated reporter gene expression was evaluated using the androgen-sensitive stable prostatic PALM cell line. Unlike POS extracts, GBL and ECS extracts showed estrogenic (0.07 and 0.20 nM E2Eq mg(-1), respectively) and anti-estrogenic (0.01 and 0.02 μM ICI182780Eq mg(-1), respectively) activities. ECS extracts evidenced androgenic activity (0.30 nM R1881Eq mg(-1)) and POS extracts anti-androgenic activity (22.30 μM ProcEq mg(-1)). According to these findings, these plant extracts may interfere with the endocrine system via one or more hormonal receptors, and further investigation is warranted into their role as endocrine disrupters in humans
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Plantas medicinales
Andrógenos
Ensayos biológicos
Assessment of hormone-like activities in Ginkgo biloba, Elettaria cardamomum and Plantago ovata extracts using in vitro receptor-specific bioassays
journal article
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