2026-05-28T19:44:29Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/343522026-02-03T11:13:41Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Minina, Elena A author Filonova, Lada H author Fukada, Kazutake author Savenkov, Eugene I author Gogvadze, Vladimir author Clapham, David author Sánchez-Vera, Victoria author Suárez-Marín, María Fernanda author Zhivotovsky, Boris author Daniel, Geoffrey author Smertenko, Andrei author Bozhkov, Peter V author 2013-12-16 Although animals eliminate apoptotic cells using macrophages, plants use cell corpses throughout development and disassemble cells in a cell- autonomous manner by vacuolar cell death. During vacuolar cell death, lytic vacuoles gradually engulf and digest the cytoplasmic content. On the other hand, acute stress triggers an alternative cell death, necrosis, which is char- acterized by mitochondrial dysfunction, early rupture of the plasma membrane, and disordered cell disassembly. How both types of cell death are regulated remains ob- scure. In this paper, we show that vacuolar death in the embryo suspensor of Norway spruce requires autophagy. In turn, activation of autophagy lies downstream of metacaspase mcII-Pa, a key protease essential for suspensor cell death. Genetic suppression of the metacaspase– autophagy pathway induced a switch from vacuolar to necrotic death, resulting in failure of suspensor differentiation and embryonic arrest. Our results establish metacaspase- dependent autophagy as a bona fide mechanism that is responsible for cell disassembly during vacuolar cell death and for inhibition of necrosis. Elena A. Minina, Lada H. Filonova, Kazutake Fukada, Eugene I. Savenkov, Vladimir Gogvadze, David Clapham, Victoria Sanchez-Vera, Maria F. Suarez, Boris Zhivotovsky, Geoffrey Daniel, Andrei Smertenko, Peter V. Bozhkov; Autophagy and metacaspase determine the mode of cell death in plants. J Cell Biol 23 December 2013; 203 (6): 917–927. doi: https://doi.org/10.1083/jcb.201307082 https://hdl.handle.net/10630/34352 10.1083/jcb.201307082 Células - Muerte Vacuolas vegetales Autophagy and metacaspase determine the mode of cell death in plants