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00925njm 22002777a 4500 dc Ruiz-López, Noemí author Pérez-Sancho, Jessica author Esteban del Valle, Alicia author Haslam, Richard P. author Vanneste, Steffen author Catalá, Rafael author Perea-Resa, Carlos author Van Damme, Daniël author García-Hernández, Selene author Albert, Armando author Vallarino Castro, José G. author Lin, Jinxing author Friml, Jiří author Macho, Alberto P author Salinas, Julio author Rosado, Abel author Napier, Johnathan A. author Amorim-Silva, Vitor author Botella-Mesa, Miguel Ángel author 2021-05-04 Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild-type while the levels of most glycerolipid species remain unchanged. In addition, the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress. Noemi Ruiz-Lopez, Jessica Pérez-Sancho, Alicia Esteban del Valle, Richard P. Haslam, Steffen Vanneste, Rafael Catalá, Carlos Perea-Resa, Daniël Van Damme, Selene García-Hernández, Armando Albert, José Vallarino, Jinxing Lin, Jiří Friml, Alberto P. Macho, Julio Salinas, Abel Rosado, Johnathan A. Napier, Vitor Amorim-Silva, Miguel A. Botella, Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress, The Plant Cell, Volume 33, Issue 7, July 2021, Pages 2431–2453, https://doi.org/10.1093/plcell/koab122 https://hdl.handle.net/10630/34509 10.1093/plcell/koab122 Membrana celular Proteínas de membranas Retículo endoplásmico Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress