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Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform. Labella Vera, Alejandro Manuel Gémez Mata, Juan Vera, Isabel Bandin, Isabel Leiva Rebollo, Rocío Borrego-García, Juan José García-Rosado, Esther Peces - Agentes patógenos Nervous necrosis virus (NNV) is the causative agent of the viral encephalopathy and retinopathy, a disease that affects cultured Senegalese sole (Solea senegalensis). A NNV reassortant (Ss160.03), combining genomic segments from red-spotted grouper nervous necrosis virus (RGNNV) and striped jack nervous necrosis virus (SJNNV) genotypes, has been previously isolated from Senegalese sole, being highly virulent to this fish species. The RNA-Seq technology has been used in a previous study to comparatively analyse Senegalese sole transcriptomes in two organs (head kidney and eye/brain) after infection with two NNV virus with different levels of virulence to that fish species, a highly virulent reassortant isolate (wSs160.03) and a less virulent mutant reassortant obtained by reverse genetics (rSs160.03247þ270). To validate previous RNA-Seq results, a 112- assay OpenArray® platform (Thermofisher) has been designed. This platform included 89 genes chosen according to transcriptomic changes observed by RNA-Seq (covering PRRs, type I IFN response, signal transduction, inflammation, virus responsive genes, and apoptosis), 17 genes selected based on their previously described relation with the immune response against fish viral infections, and 6 control genes (including 3 endogenous genes and 3 viral genes). 2024-10-10T08:42:05Z 2024-10-10T08:42:05Z 2024-10-10T08:42:05Z 2019 conference output A. Labella, J. Gémez, I. Vera, I. Bandín, R. Leiva, J.J. Borrego, E. Garcia-Rosado, Analysis of gene expression in nodavirus-inoculated Senegalese sole using a new Openarray® platform, Fish & Shellfish Immunology, Volume 91, 2019, Pages 391-392, ISSN 1050-4648, https://doi.org/10.1016/j.fsi.2019.04.077. (https://www.sciencedirect.com/science/article/pii/S1050464819303158) https://hdl.handle.net/10630/34600 10.1016/j.fsi.2019.04.077 eng International Conference on Fish and Shellfish Immunology Las Palmas de Gran Canaria, España 16/06/2019 http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier