2026-05-30T10:25:49Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/346162026-02-03T11:32:22Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Labella Vera, Alejandro Manuel
author
Arahal, David
author
Lucena, Teresa
author
Manchado, Manuel
author
Castro-López, María Dolores
author
Borrego-García, Juan José
2024-10-10T09:50:13Z
2024-10-10T09:50:13Z
2017
Labella AM, Arahal DR, Lucena T, Manchado M, Castro D, Borrego JJ. Photobacterium toruni sp. nov., a bacterium isolated from diseased farmed fish. Int J Syst Evol Microbiol. 2017 Nov;67(11):4518-4525. doi: 10.1099/ijsem.0.002325. Epub 2017 Sep 21. PMID: 28933324.
https://hdl.handle.net/10630/34616
10.1099/ijsem.0.002325
Three bacterial strains were isolated from liver and spleen of diseased farmed redbanded seabream (Pagrus auriga) in
south-west Spain. Their partial 16S rRNA gene sequences clustered within those of the genus Photobacterium, showing high
similarity (98.6–99.3 %) to the type strains of Photobacterium iliopiscarium, P. piscicola, P. kishitanii, P. aquimaris and
P. phosphoreum. Multilocus sequence analysis using six housekeeping genes (gapA, topA, mreB, ftsZ, gyrB and 16S rRNA)
confirmed the new strains as forming an independent branch with a bootstrap value of 100, likely to represent a novel
species. To confirm this, we used whole genome sequencing and genomic analysis (ANIb, ANIm and in silico DNA–DNA
hybridization) obtaining values well below the thresholds for species delineation. In addition, a phenotypic characterization
was performed to support the description and differentiation of the novel strains from related taxa. Cells were Gram-stainnegative,
motile bacilli, chemo-organotrophic and facultatively anaerobic. They fermented glucose, as well as galactose and
D-mannose, without production of gas. Oxidase and catalase were positive. The predominant cellular fatty acids were C16 : 1
!7c/C16 : 1!6c and C16 : 0. The predominant respiratory quinone (Q-8) and major polar lipids (phosphatidylethanolamine,
phosphatidylglycerol and diphosphatidylglycerol) were inferred from annotated genes in the genome of strain H01100410BT,
which had a G+C content of 38.6 mol%. The results obtained demonstrate that the three strains represent a novel species,
for which the name Photobacterium toruni sp. nov. is proposed. The type strain is H01100410BT (=CECT 9189T=LMG 29991T).
eng
Atribución 4.0 Internacional
Piscicultura - Aspectos genéticos
Photobacterium toruni sp. nov., a bacterium isolated from diseased farmed fish.
journal article
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15. Labella et al., 2017.pdf