2026-05-28T13:12:47Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/352732026-02-03T11:29:00Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Fernández-Arjona, María del Mar
author
León-Rodríguez, Ana
author
López-Ávalos, María Dolores
author
Mateos-Grondona, Jesús
2024-11-25T09:51:10Z
2024-11-25T09:51:10Z
2021-03-23
Fernández-Arjona, M.d., León-Rodríguez, A., López-Ávalos, M.D. et al. Microglia activated by microbial neuraminidase contributes to ependymal cell death. Fluids Barriers CNS 18, 15 (2021). https://doi.org/10.1186/s12987-021-00249-0
https://hdl.handle.net/10630/35273
10.1186/s12987-021-00249-0
The administration of microbial neuraminidase into the brain ventricular cavities of rodents represents a model of
acute aseptic neuroinflammation. Ependymal cell death and hydrocephalus are unique features of this model. Here
we demonstrate that activated microglia participates in ependymal cell death. Co-cultures of pure microglia with
ependymal cells (both obtained from rats) were performed, and neuraminidase or lipopolysaccharide were used
to activate microglia. Ependymal cell viability was unaltered in the absence of microglia or inflammatory stimulus
(neuraminidase or lipopolysaccharide). The constitutive expression by ependymal cells of receptors for cytokines
released by activated microglia, such as IL-1β, was demonstrated by qPCR. Besides, neuraminidase induced the overexpression
of both receptors in ventricular wall explants. Finally, ependymal viability was evaluated in the presence of
functional blocking antibodies against IL-1β and TNFα. In the co-culture setting, an IL-1β blocking antibody prevented
ependymal cell death, while TNFα antibody did not. These results suggest that activated microglia are involved in the
ependymal damage that occurs after the administration of neuraminidase in the ventricular cavities, and points to
IL-1β as possible mediator of such effect. The relevance of these results lies in the fact that brain infections caused by
neuraminidase-bearing pathogens are frequently associated to ependymal death and hydrocephalus.
eng
Attribution 4.0 Internacional
Sistema nervioso - Inflamación
Monosacáridos
Neuroglia
Células - Muerte
Microglia activated by microbial neuraminidase contributes to ependymal cell death.
journal article
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Fernández-Arjona 2021 Microglia activated by microbial neuraminidase contributes to ependymal cell death_Barriers and Fluids of the CNS.pdf