2026-05-30T04:03:15Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/353142026-02-03T11:11:49Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Domínguez Maqueda, Marta author Pérez Gómez, Olivia author García Márquez, Jorge author Espinosa Ruíz, Cristóbal author Cuesta, Alberto author Esteban, Mª. Ángeles author Alarcón López, Francisco Javier author Cárdenas, Casimiro author Tapia-Paniagua, Silvana Teresa author Balebona-Accino, María del Carmen author Moriñigo-Gutiérrez, Miguel Ángel author 2024 Postbiotics are metabolic by-products from microorganisms that provide health benefits to the host. Their secretion can be influenced by various conditions affecting bacterial metabolism. This study presents a novel ap- proach for producing potential postbiotics, specifically extracellular products (ECPs), from the probiotic strain Shewanella putrefaciens SpPdp11, grown under different culture conditions. These conditions include aquafeed media, with partial or total microalgae/cyanobacteria replacement as the microbial substrate, as well as variations in temperature and growth phase. The use of microalgae/cyanobacteria as substrates may represent a valuable strategy for generating novel postbiotics with unique properties. The ECPs assessed were evaluated for their in vitro cytotoxic, hydrolytic and antimicrobial ac- tivities. Three conditions (ECPs derived from aquafeed media with partial (FM2324 and FM1548) or total (M2324) microalgae/cyanobacteria replace- ment) were non- cytotoxic to various fish cell lines and hydrolysed key nutri- tional compounds (casein, lipids, amylase and gelatin). Proteomic analysis of these ECP conditions revealed common structural and regulatory DNA- associated proteins, while differentially expressed proteins were associated with amino acid metabolism and antioxidant system (FM2324 and FM1548) and chemotaxis system (M2324). The results highlight the potential of the selected postbiotics as feed additives for future in vivo studies, aligning with sustainable development for aquaculture. Domínguez-Maqueda M, Pérez-Gómez O, García-Márquez J, Espinosa-Ruíz C, Cuesta A, Esteban MÁ, Alarcón-López FJ, Cárdenas C, Tapia-Paniagua ST, Balebona MC, Moriñigo MÁ. Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic. Microb Biotechnol. 2024 Nov;17(11):e70046. doi: 10.1111/1751-7915.70046. PMID: 39573896; PMCID: PMC11582085. https://hdl.handle.net/10630/35314 10.1111/1751-7915.70046 Microbiología Bacterias Microorganismos Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic