2026-06-01T04:33:05Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/353142026-02-03T11:11:49Zcom_10630_2254col_10630_37953

Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic Domínguez Maqueda, Marta Pérez Gómez, Olivia García Márquez, Jorge Espinosa Ruíz, Cristóbal Cuesta, Alberto Esteban, Mª. Ángeles Alarcón López, Francisco Javier Cárdenas, Casimiro Tapia-Paniagua, Silvana Teresa Balebona-Accino, María del Carmen Moriñigo-Gutiérrez, Miguel Ángel Microbiología Bacterias Microorganismos Postbiotics are metabolic by-products from microorganisms that provide health benefits to the host. Their secretion can be influenced by various conditions affecting bacterial metabolism. This study presents a novel ap- proach for producing potential postbiotics, specifically extracellular products (ECPs), from the probiotic strain Shewanella putrefaciens SpPdp11, grown under different culture conditions. These conditions include aquafeed media, with partial or total microalgae/cyanobacteria replacement as the microbial substrate, as well as variations in temperature and growth phase. The use of microalgae/cyanobacteria as substrates may represent a valuable strategy for generating novel postbiotics with unique properties. The ECPs assessed were evaluated for their in vitro cytotoxic, hydrolytic and antimicrobial ac- tivities. Three conditions (ECPs derived from aquafeed media with partial (FM2324 and FM1548) or total (M2324) microalgae/cyanobacteria replace- ment) were non- cytotoxic to various fish cell lines and hydrolysed key nutri- tional compounds (casein, lipids, amylase and gelatin). Proteomic analysis of these ECP conditions revealed common structural and regulatory DNA- associated proteins, while differentially expressed proteins were associated with amino acid metabolism and antioxidant system (FM2324 and FM1548) and chemotaxis system (M2324). The results highlight the potential of the selected postbiotics as feed additives for future in vivo studies, aligning with sustainable development for aquaculture. 2024-11-26T11:11:40Z 2024-11-26T11:11:40Z 2024-11-26T11:11:40Z 2024 journal article Domínguez-Maqueda M, Pérez-Gómez O, García-Márquez J, Espinosa-Ruíz C, Cuesta A, Esteban MÁ, Alarcón-López FJ, Cárdenas C, Tapia-Paniagua ST, Balebona MC, Moriñigo MÁ. Microalgae and cyanobacteria as microbial substrate and their influence on the potential postbiotic capability of a bacterial probiotic. Microb Biotechnol. 2024 Nov;17(11):e70046. doi: 10.1111/1751-7915.70046. PMID: 39573896; PMCID: PMC11582085. https://hdl.handle.net/10630/35314 10.1111/1751-7915.70046 spa http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley