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oai:riuma.uma.es:10630/356752026-02-03T11:18:39Zcom_10630_2254col_10630_37953

The NAC transcription factor FaRIF controls fruit ripening in strawberry Martín-Pizarro, Carmen Vallarino, José G. Osorio-Algar, Sonia Meco, Victoriano Urrutia, María Pillet, Jeremy Casañal, Ana Merchante, Catharina Amaya, Iraida Willmitzer, Lothar Fernie, Alisdair R. Giovannoni, James J. Botella-Mesa, Miguel Ángel Valpuesta-Fernández, Victoriano Posé-Padilla, David Fresas In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling the ripening of straw- berry, a nonclimacteric fruit, is still limited. NAC transcription factors (TFs) mediate different developmental processes in plants. Here, we identified and characterized Ripening Inducing Factor (FaRIF), a NAC TF that is highly expressed and in- duced in strawberry receptacles during ripening. Functional analyses based on stable transgenic lines aimed at silencing FaRIF by RNA interference, either from a constitutive promoter or the ripe receptacle-specific EXP2 promoter, as well as overexpression lines showed that FaRIF controls critical ripening-related processes such as fruit softening and pigment and sugar accumulation. Physiological, metabolome, and transcriptome analyses of receptacles of FaRIF-silenced and overexpres- sion lines point to FaRIF as a key regulator of strawberry fruit ripening from early developmental stages, controlling abscisic acid biosynthesis and signaling, cell-wall degradation, and modification, the phenylpropanoid pathway, volatiles production, and the balance of the aerobic/anaerobic metabolism. FaRIF is therefore a target to be modified/edited to control the qual- ity of strawberry fruits. 2024-12-13T12:09:51Z 2024-12-13T12:09:51Z 2021-02-24 journal article Carmen Martín-Pizarro, José G Vallarino, Sonia Osorio, Victoriano Meco, María Urrutia, Jeremy Pillet, Ana Casañal, Catharina Merchante, Iraida Amaya, Lothar Willmitzer, Alisdair R Fernie, James J Giovannoni, Miguel A Botella, Victoriano Valpuesta, David Posé, The NAC transcription factor FaRIF controls fruit ripening in strawberry, The Plant Cell, Volume 33, Issue 5, May 2021, Pages 1574–1593, https://doi.org/10.1093/plcell/koab070 https://hdl.handle.net/10630/35675 10.1093/plcell/koab070 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Oxford University Press