2026-05-27T04:42:56Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/357052026-02-03T11:25:59Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
López-Márquez, Diego
author
Del Espino Pérez, Ángel
author
López-Pagán, Nieves
author
Rodrígez-Negrete, Edgar A.
author
Rubio-Somoza, Ignacio
author
Ruiz-Albert, Francisco Javier
author
Rodríguez-Bejarano, Eduardo
author
Beuzón-López, Carmen del Rosario
2024-12-17T09:55:01Z
2024-12-17T09:55:01Z
2021-10-26
López-Márquez D, Del-Espino Á, López-Pagán N, Rodríguez-Negrete EA, Rubio-Somoza I, Ruiz-Albert J, Bejarano ER, Beuzón CR. miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis. J Exp Bot. 2021 Oct 26;72(20):7316-7334
https://hdl.handle.net/10630/35705
10.1093/jxb/erab354
Plants encode numerous intracellular receptors known as nucleotide-binding leucine-rich repeat receptors (NLRs)
that recognize pathogen-derived effectors or their activity to activate defenses. miRNAs regulate NLR genes in many
species, often triggering the production of phased siRNAs (phasiRNAs). Most such examples involve genes encoding
NLRs carrying coiled-coil domains, although a few include genes encoding NLRs carrying a Toll/interleukin-1 domain
(TNL). Here, we characterize the role of miR825-5p in Arabidopsis, using a combination of bioinformatics, transgenic
plants with altered miRNA levels and/or reporters, small RNAs, and virulence assays. We demonstrate that
miR825-5p down-regulates the TNL MIST1 by targeting for endonucleolytic cleavage the sequence coding for TIR2,
a highly conserved amino acid motif, linked to a catalytic residue essential for immune function. miR825-5p acts as
a negative regulator of basal resistance against Pseudomonas syringae. miR825-5p triggers the production from
MIST1 of a large number of phasiRNAs that can mediate cleavage of both MIST1 and additional TNL gene transcripts,
potentially acting as a regulatory hub. miR825-5p is expressed in unchallenged leaves and transcriptionally downregulated
in response to pathogen-associated molecular patterns (PAMPs). Our results show that miR825-5p, which
is required for full expression of PAMP-triggered immunity, establishes a link between PAMP perception and expression
of uncharacterized TNL genes.
eng
Attribution-NoDerivatives 4.0 Internacional
Receptores celulares
miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis
journal article
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