2026-05-30T15:50:42Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/358702026-02-03T11:13:39Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Nualart, Francisco author Cifuentes-Rueda, Manuel author Ramírez, Eder author Martínez, Fernando author Barahona, M José author Ferrada, Luciano author Saldivia, N author Borgonzone, Ernesto R author Thorens, B author Salazar, Katterine author 2023-09 Hyperglycemia increases glucose concentrations in the cerebrospinal fluid (CSF), activating glucose-sensing mechanisms and feeding behavior in the hypothalamus. Here, we discuss how hyperglycemia temporarily modifies ependymal cell ciliary beating to increase hypothalamic glucose sensing. A high level of glucose in the rat CSF stimulates glucose transporter 2 (GLUT2)-positive subcommissural organ (SCO) cells to release SCO-spondin into the dorsal third ventricle. Genetic inactivation of mice GLUT2 decreases hyperglycemia-induced SCO-spondin secretion. In addition, SCO cells secrete Wnt5a-positive vesicles; thus, Wnt5a and SCO-spondin are found at the apex of dorsal ependymal cilia to regulate ciliary beating. Frizzled-2 and ROR2 receptors, as well as specific proteoglycans, such as glypican/testican (essential for the interaction of Wnt5a with its receptors) and Cx43 coupling, were also analyzed in ependymal cells. Finally, we propose that the SCO-spondin/Wnt5a/Frizzled-2/Cx43 axis in ependymal cells regulates ciliary beating, a cyclic and adaptive signaling mechanism to control glucose sensing. Nualart F, Cifuentes M, Ramírez E, Martínez F, Barahona MJ, Ferrada L, Saldivia N, Bongarzone ER, Thorens B, Salazar K. Hyperglycemia increases SCO-spondin and Wnt5a secretion into the cerebrospinal fluid to regulate ependymal cell beating and glucose sensing. PLoS Biol. 2023 Sep 21;21(9):e3002308. doi: 10.1371/journal.pbio.3002308. PMID: 37733692; PMCID: PMC10513282. https://hdl.handle.net/10630/35870 https://doi.org/10.1371/journal.pbio.3002308 Glucemia Hyperglycemia increases SCO-spondin and Wnt5a secretion into the cerebrospinal fluid to regulate ependymal cell beating and glucose sensing