2026-05-31T04:01:14Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/361012026-02-03T10:52:20Zcom_10630_2254col_10630_37953

Rapid amplitude-modulation of a diarylethene photoswitch: En route to contrast-enhanced fluorescence imaging Naren, Gaowa Larsson, Wera Benítez-Martín, Carlos Li, Shiming Perez-Inestrosa, Ezequiel Albinsson, Bo Andréasson, Joakim Fluorescencia Fluorescence imaging Diarylethene Contrast-enhanced A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy Swedish Research Council VR Spanish Ministry for Science, Innovation, and Universities Universidad de Málaga-Junta de Andalucía FEDER 2025-01-10T08:07:35Z 2025-01-10T08:07:35Z 2021 journal article VoR Rapid amplitude-modulation of a diarylethene photoswitch: En route to contrast-enhanced fluorescence imaging Gaowa Naren, Wera Larsson, Carlos Benitez-Martin, Shiming Li, Ezequiel Perez-Inestrosa, Bo Albinsson and Joakim Andréasson Chemical Science, 2021, 12, 7073-7078. DOI: 10.1039/D1SC01071A https://hdl.handle.net/10630/36101 10.1039/D1SC01071A eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf The Royal Society of Chemistry