2026-06-02T01:24:58Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/363222026-02-03T11:03:34Zcom_10630_2254col_10630_37953
Indolenine-Based Derivatives as Customizable Two-Photon Fluorescent Probes for pH Bioimaging in Living Cells
Benítez-Martín, Carlos
Guadix-Domínguez, Juan Antonio
Pearson, John
Nájera-Albendín, Francisco
Pérez-Pomares, José María
Perez-Inestrosa, Ezequiel
Detectores de fotones
Intracellular pH
pH sensor
Two-photon absorption
Fluorescent probe
Biosensor
Novel pH probes based on 2-(6-methoxynaphthalen-2-yl)-3,3-dimethyl3H-indole have been synthesized and characterized. These compounds display excellent
“off−on” fluorescence responses to acidic pH especially under two-photon (TP) excitation
conditions as well as strong selectivity and sensitivity toward H+
. These features are
supported by fluorescence quantum yields over 35%, TP cross sections ∼60 GM, and
good resistance to photodegradation under acidic conditions. The synthetic versatility of
this model allows subcellular targets to be tuned through minor scaffold modifications
without affecting its optical characteristics. The effectiveness of the probes’ innate
photophysical properties and the structural modifications for different pH-related
applications are demonstrated in mouse embryonic fibroblast cells
Spanish Ministerio de Ciencia, Innovación y Universidades
Consejería de Salud, Junta de Andalucía
Universidad de Málaga-Junta de Andalucía
FEDER funds
2025-01-15T07:56:42Z
2025-01-15T07:56:42Z
2020
journal article
AM
Carlos Benitez-Martin, Juan A. Guadix, John R. Pearson, Francisco Najera, Jose M. Perez-Pomares, and Ezequiel Perez-Inestrosa ACS Sensors 2020 5 (4), 1068-1074 DOI: 10.1021/acssensors.9b02590
https://hdl.handle.net/10630/36322
10.1021/acssensors.9b02590
eng
open access
application/pdf
American Chemical Society