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oai:riuma.uma.es:10630/369192026-02-03T11:00:45Zcom_10630_2254col_10630_37953

Nieto-Torres, Jose Luis López-De-Diego, Marta Álvarez-Coruña, Enrique Jimenez-Guardeño, Jose Manuel Regla-Nava, Jose Angel Llorente-Gómez, Mercedes Kremer, Leonor Shuo, Shen Enjuanes, Luis 2025-01-24T11:24:27Z 2025-01-24T11:24:27Z 2011 Jose L. Nieto-Torres, Marta L. DeDiego, Enrique Álvarez, Jose M. Jiménez-Guardeño, Jose A. Regla-Nava, Mercedes Llorente, Leonor Kremer, Shen Shuo, Luis Enjuanes, Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein, Virology, Volume 415, Issue 2, 2011, Pages 69-82, ISSN 0042-6822, https://doi.org/10.1016/j.virol.2011.03.029 https://hdl.handle.net/10630/36919 10.1016/j.virol.2011.03.029 Severe acute respiratory syndrome (SARS) coronavirus (CoV) envelope (E) protein is a transmembrane protein. Several subcellular locations and topological conformations of E protein have been proposed. To identify the correct ones, polyclonal and monoclonal antibodies specific for the amino or the carboxy terminus of E protein, respectively, were generated. E protein was mainly found in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of cells transfected with a plasmid encoding E protein or infected with SARS-CoV. No evidence of E protein presence in the plasma membrane was found by using immunofluorescence, immunoelectron microscopy and cell surface protein labeling. In addition, measurement of plasma membrane voltage gated ion channel activity by whole-cell patch clamp suggested that E protein was not present in the plasma membrane. A topological conformation in which SARS-CoV E protein amino terminus is oriented towards the lumen of intracellular membranes and carboxy terminus faces cell cytoplasm is proposed. spa http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Coronavirus COVID-19 Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein journal article