2026-06-01T10:17:42Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/373372026-02-03T10:52:33Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Perán-Quesada, Rosa Amelia
author
Sánchez-Romero, Carolina
author
Barcelo-Muñoz, Araceli
author
Pliego-Alfaro, Fernando
2025-01-29T19:07:24Z
2025-01-29T19:07:24Z
2004
R. Perán-Quesada, C. Sánchez-Romero, A. Barceló-Muñoz, F. Pliego-Alfaro, Factors affecting maturation of avocado somatic embryos, 2004. Scientia Horticulturae, Vol 102, Issue 1, Pp 61-73, ISSN 0304-4238. https://doi.org/10.1016/j.scienta.2003.12.003.
https://hdl.handle.net/10630/37337
10.1016/j.scienta.2003.12.003
The effect of mineral salts, sucrose, gellan gum, abscisic acid and coconut water on maturation of avocado (Persea americana Mill.) somatic embryos was studied. Use of B5 major salts was essential to obtain white-opaque embryos. Sucrose at 175 mM, gellan gum (6.8 g l−1 ) or coconut water (10–20%) also enhanced the recovery of white-opaque embryos. Abscisic acid slightly enhanced the appearance of white-opaque embryos. White-opaque embryos were further grown on MS, modified B5 medium
supplemented with 10% coconut water or Jensen–MS media and they subsequently germinated on M1 medium. Higher germination rates (11.11%) were obtained with embryos that matured on modified B5 medium supplemented with 10% coconut water, although larger embryos developed on MS medium. Thus, embryo size during maturation does not seem to be correlated with germination capacity. Shoots obtained from germinated embryos could be multiplied in the presence of 4.44 M benzylaminopurine and successfully rooted (80% rate) after a 3 days treatment with 4.92 M indole-3-butyric acid. Over
90% of the rooted shoots survived acclimatization.
eng
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Aguacates
Factors affecting maturation of avocado somatic embryos
journal article
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Preprint_2004_Factors affecting maturation of avocado somatic embryos.pdf