2026-05-29T23:01:10Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/374622026-02-03T11:26:22Zcom_10630_2254col_10630_37953

Global transcriptional response of solvent‐sensitive and solvent‐tolerant Pseudomonas putida strains exposed to toluene. Molina-Santiago, Carlos Udaondo, Zulema Gómez-Lozano, María Molin, Soren Ramos, Juan Luis Tolueno Pseudomonas putida strains are generally recognized as solvent tolerant, exhibiting varied sensitivity to organic solvents. Pan-genome analysis has revealed that 30% of genes belong to the core-genome of Pseudomonas. Accessory and unique genes confer high degree of adaptability and capabilities for the degradation and synthesis of a wide range of chemicals. For the use of these microbes in bioremediation and biocatalysis, it is critical to understand the mechanisms underlying these phenotypic differences. In this study, RNA-seq analysis compared the short- and long-term responses of the toluene-sensitive KT2440 strain and the highly tolerant DOT-T1E strain. The sensitive strain activates a larger number of genes in a higher magnitude than DOT-T1E. This is expected because KT2440 bears one toluene tolerant pump, while DOT-T1E encodes three of these pumps. Both strains activate membrane modifications to reduce toluene membrane permeability. The KT2440 strain activates the TCA cycle to generate energy, while avoiding energy-intensive processes such as flagellar biosynthesis. This suggests that KT2440 responds to toluene by focusing on survival mechanisms. The DOT-T1E strain activates toluene degradation pathways, using toluene as source of energy. Among the unique genes encoded by DOT-T1E is a 70 kb island composed of genes of unknown function induced in response to toluene. 2025-01-31T07:33:44Z 2025-01-31T07:33:44Z 2025-01-31T07:33:44Z 2016 journal article Molina-Santiago, C., Udaondo, Z., Gómez-Lozano, M., Molin, S. and Ramos, J.-L. (2017), Global transcriptional response of solvent-sensitive and solvent-tolerant Pseudomonas putida strains exposed to toluene. Environ Microbiol, 19: 645-658. https://doi.org/10.1111/1462-2920.13585 https://hdl.handle.net/10630/37462 10.1111/1462-2920.13585 eng open access Wiley