2026-05-31T16:16:22Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/376212026-02-23T10:49:23Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Castellano-Castillo, Daniel
author
Moreno-Indias, Isabel
author
Picón-César, María José
author
Garcia-Aleman, Jorge
author
Macías-González, Manuel
author
Tinahones-Madueño, Francisco José
author
Queipo-Ortuño, María Isabel
author
Cardona-Díaz, Fernando
2025-02-03T10:25:24Z
2025-02-03T10:25:24Z
2014-08-01
Asociation between metabolic status and the methylation level of genes involved in metabolic disorders and obesity Castellano-Castillo, D. et al. Atherosclerosis, Volume 235, Issue 2, e128
https://hdl.handle.net/10630/37621
Objectives: DNA methylation is one of the epigenetic mechanims to control gene expression, and it is believed to be modulated by environmental and nutritional factors. Thus, epigenetics provides a mechanism which may explain the etiology of type 2 diabetes and obesity.
The aim of this study is to analyse the methylation level in visceral adipose tissue (VAT) of PPARa, RXRa, LPL and LEP, genes related to the HOMAIR and BMI.
Methods: A total of 61 VAT samples were obtained during bariatric or hiatal hernia surgeries. Biochemical parameters from these patients were measured. Samples were assigned to four groups attending their BMI:: normalweigh, overweigh, obese and morbidly obese (<25, 25.1-30, 30.1- 40, >40.1). Furthermore, the patients were classified in two groups ac- cording to the HOMA-IR (HOMA-IR<4 and HOMA-IR 4.1-7.5). Methylation level was measured through pyrosequentation, using the Pyromark tech- nology and predesigned CpG methylation assays (Qiagen). The results were analyzed using the CpG methylation software (Qiagen) and the sta- tistical package SPSS.
Results: We found a negative correlation between RXRa methylation level and the BMI. In the case of PPARa existed a positive correlation with the HOMA-IR, while LPL correlated positively with both, preprandial and postprandial triglycerid levels, and with the HOMA-IR. Furthermore, we found significative differences in the methylation levels of PPARa by BMI and HOMA-IR, and by HOMA-IR for LPL
Conclusion: A relationship exists between the methylation level of the studied genes and the HOMA-IR and BMI. Moreover,there is an association of PPARa with the BMI and LPL with the metabolic state.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Epigenética
Association between metabolic status and the methylation level of genes involved in metabolic disorders and obesity
conference output
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