2026-05-31T21:03:48Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/379192026-02-03T11:03:35Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
El-Azaz-Ciudad, Jorge
author
De-la-Torre-Fazio, Fernando Nicolás
author
Pascual-Moreno, María Belén
author
Debille, Sandrine
author
Harvengt, Luc
author
Trontin, Jean-François
author
Ávila-Sáez, Concepción
author
Cánovas-Ramos, Francisco Miguel
2025-02-18T12:00:03Z
2025-02-18T12:00:03Z
2020
Jorge El-Azaz, Fernando de la Torre, María Belén Pascual, Sandrine Debille, Francis Canlet, Luc Harvengt, Jean-François Trontin, Concepción Ávila, Francisco M Cánovas, Transcriptional analysis of arogenate dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis, Journal of Experimental Botany, Volume 71, Issue 10, 30 May 2020, Pages 3080–3093, https://doi.org/10.1093/jxb/eraa099
0022-0957
https://hdl.handle.net/10630/37919
10.1093/jxb/eraa099
Biogenesis of the secondary cell wall in trees involves the massive biosynthesis of the phenylalanine-derived polymer lignin. Arogenate dehydratase (ADT) catalyzes the last, and rate-limiting, step of the main pathway for phenylalanine biosynthesis. In this study, we found that transcript levels for several members of the large ADT gene family, including ADT-A and ADT-D, were enhanced in compression wood of maritime pine, a xylem tissue enriched in lignin. Transcriptomic analysis of maritime pine silenced for PpMYB8 revealed that this gene plays a critical role in coordinating the deposition of lignin with the biosynthesis of phenylalanine. Specifically, it was found that ADT-A and ADT-D were strongly down-regulated in PpMYB8-silenced plants and that they were transcriptionally regulated through direct interaction of this transcription factor with regulatory elements present in their promoters. Another transcription factor, PpHY5, exhibited an expression profile opposite to that of PpMYB8 and also interacted with specific regulatory elements of ADT-A and ADT-D genes, suggesting that it is involved in transcriptional regulation of phenylalanine biosynthesis. Taken together, our results reveal that PpMYB8 and PpHY5 are involved in the control of phenylalanine formation and its metabolic channeling for lignin biosynthesis and deposition during wood formation in maritime pine.
eng
Biosíntesis
Genetica vegetal
Fenilalanina
Lignina
Transcriptional analysis of arogenato dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis.
journal article
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URL
https://riuma.uma.es/bitstreams/6f9ef9fd-3d98-4a7e-b1ac-6f627c07b7e0/download
File
MD5
7c80a49b7247afb4eaf62e8d7002928d
2656903
application/pdf
El-Azaz J Exp Bot 2020.pdf