2026-06-01T19:50:17Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/379702026-02-03T10:52:29Zcom_10630_2254col_10630_37953

Caballano-Infantes, Estefanía García-García, Alberto Lopez-Gomez, Carlos Cueto-Sánchez, Alejandro Robles-Díaz, María Mercedes Ortega-Alonso, Aida Martín-Reyes, Flores Álvarez-Álvarez, Ismael Arranz-Salas, Isabel María Ruiz-Cabello, Francisco Lucena-González, María Isabel García-Fuentes, Eduardo Andrade-Bellido, Raúl Jesús García-Cortés, Miren 2025-02-20T12:02:50Z 2025-02-20T12:02:50Z 2021-01-28 Caballano-Infantes E, García-García A, Lopez-Gomez C, Cueto A, Robles-Diaz M, Ortega-Alonso A, Martín-Reyes F, Alvarez-Alvarez I, Arranz-Salas I, Ruiz-Cabello F, Lucena IM, García-Fuentes E, Andrade RJ, García-Cortes M. Differential iNKT and T cells activation in non-alcoholic fatty liver disease and drug-induced liver injury. Biomedicines. 2021;10(1):55 https://hdl.handle.net/10630/37970 10.3390/biomedicines10010055 Background: Non-alcoholic fatty liver disease (NAFLD) and idiosyncratic drug-induced liver injury (DILI) could share molecular mechanisms involving the immune system. We aimed to identify activation immunological biomarkers in invariant natural killer T (iNKT) and CD4/CD8+ T cells in NAFLD and DILI. Methods: We analyzed the activation profile (CD69, CD25, and HLA-DR) and natural killer group 2 member D (NKG2D) on iNKT cells, and CD4/CD8 T cells in peripheral blood mononuclear cells from NAFLD, with or without significant liver fibrosis, and DILI patients. Results: There was an increase in iNKT cells in NAFLD patients compared to DILI or control subjects. Regarding the cellular activation profile, NAFLD with significant liver fibrosis (F ≥ 2) displayed higher levels of CD69+iNKT cells compared to NAFLD with none or mild liver fibrosis (F ≥ 1) and control patients. CD69+iNKT positively correlated with insulin resistance, aspartate aminotransferase (AST) level, liver fibrosis-4 index (FIB4) and AST to Platelet Ratio Index (APRI). DILI patients showed an increase in CD69+ and HLA-DR+ in both CD4+ and CD8+ T cells, detecting the most relevant difference in the case of CD69+CD8+ T cells. Conclusions: CD69+iNKT may be a biomarker to assess liver fibrosis progression in NAFLD. CD69+CD8+ T cells were identified as a potential distinctive biomarker for distinguishing DILI from NAFLD. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Hígado - Enfermedades Hígado - Fibrosis Inmunohematología Efectos indeseables de los medicamentos Differential iNKT and T cells activation in non-alcoholic fatty liver disease and drug-induced liver injury. journal article