2026-06-01T12:31:21Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/386132026-02-03T11:34:28Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Garzarella, Emanuele Ugo author Navajas Porras, Beatriz author Pérez-Burillo, Sergio author Ullah, Hammad author Esposito, Cristina author Santarcangelo, Cristina author Hinojosa Nogueira, Daniel author Pastoriza, Silvia author Zaccaria, Vincenzo author Xiao, Jianbo author Rufián-Henares, José Ángel author Daglia, Maria author 2022-04 Introduction: A large body of evidence suggests that propolis exerts antioxidant, anti-inflammatory, and antimicrobial activities, mostly ascribed to its polyphenol content. Growing evidence suggests that propolis could modulate gut microbiota exerting a positive impact on several pathological conditions. The aim of this study was to determine the in vitro impact of a poplar-type propolis extract with a standardized polyphenol content, on the composition and functionality of gut microbiota obtained from fecal material of five different donors (healthy adults, and healthy, obese, celiac, and food allergic children). Methods: The standardized polyphenol mixture was submitted to a simulated in vitro digestion-fermentation process, designed to mimic natural digestion in the human oral, gastric, and intestinal chambers. The antioxidant profile of propolis before and after the digestion-fermentation process was determined. 16 S rRNA amplicon next-generation sequencing (NGS) was used to test the effects on the gut microbiota of propolis extract. The profile of the short-chain fatty acids (SCFA) produced by the microbiota was also investigated through a chromatographic method coupled with UV detection. Results: In vitro digestion and fermentation induced a decrease in the antioxidant profile of propolis (i.e., decrease of total polyphenol content, antiradical and reducing activities). Propolis fermentation exhibited a modulatory effect on gut microbiota composition and functionality of healthy and diseased subjects increasing the concentration of SCFA. Conclusions: Overall, these data suggest that propolis might contribute to gut health and could be a candidate for further studies in view of its use as a prebiotic ingredient. Garzarella EU, Navajas-Porras B, Pérez-Burillo S, Ullah H, Esposito C, Santarcangelo C, et al. Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota. Biomedicine & Pharmacotherapy. 2022;148:112759. https://hdl.handle.net/10630/38613 10.1016/j.biopha.2022.112759 Acidos grasos Digestión Antioxidantes Flora intestinal Propóleos Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota.