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oai:riuma.uma.es:10630/386572026-02-03T11:20:29Zcom_10630_2254col_10630_37953

Anti‑nervous necrosis virus activity of the growth hormone releasing peptide‑6, GHRP‑6 Álvarez-Torres, Daniel Martínez, Rebeca Moreno, Patricia Alonso-Sánchez, María del Carmen García-Rosado, Esther Porras Estrada, Mario Béjar-Alvarado, Julia Hormona liberadora de la somatropina Peces Agentes antivíricos Growth hormone-releasing peptide 6 (GHRP-6), is a six-amino acid synthetic peptide that acts as a ghrelin analog and has shown growth-promoting and immunomodulatory activities in several fish species. This study evaluates the effects of GHRP-6 against the infection with the nervous necrosis virus (NNV), an important pathogen affecting the central nervous system of a wide range of fish species, including European seabass (Dicentrarchus labrax). For the in vitro evaluation, E11 cells were treated with GHRP-6 before and/or during viral infection, resulting in higher cellular survival rates, lower viral genome replication, and lower production of infective viral particles compared with control cells. These results indicate that GHRP-6 protects E11 cells from the viral infection. In order to conduct the in vivo evaluation, European seabass specimens were intraperitoneally injected with GHRP-6, resulting in the significant upregulation of several immune genes, such as TNF-A, RTP3, and IgM, in head kidney and intestine. Furthermore, NNV replication was lower in the brain from GHRP-6 injected fish than in controls, and the brain antiviral immune response was clearly modulated in treated fish. These results support the potential of GHRP-6 as an antiviral compound that could contribute to prevent viral diseases in fish farms, a priority for the aquaculture Industry 2025-05-16T11:02:49Z 2025-05-16T11:02:49Z 2025-05-16T11:02:49Z 2025-04-30 journal article Álvarez-Torres, D., Martínez, R., Moreno, P. et al. Anti-nervous necrosis virus activity of the growth hormone releasing peptide-6, GHRP-6. Aquacult Int 33, 306 (2025). https://doi.org/10.1007/s10499-025-01987-w https://hdl.handle.net/10630/38657 10.1007/s10499-025-01987-w eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Springer Nature