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oai:riuma.uma.es:10630/388292026-02-03T11:32:26Zcom_10630_2254col_10630_37953

Can, Handan Chanumolu, Sree K. González-Muñoz, María Elena Prukudom, Sukumal Otu, Hasan H. Cibelli, Jose B 2025-06-03T12:15:27Z 2025-06-03T12:15:27Z 2020-07-08 Can, H., Chanumolu, S.K., Gonzalez-Muñoz, E. et al. Comparative analysis of single-cell transcriptomics in human and zebrafish oocytes. BMC Genomics 21, 471 (2020). https://doi.org/10.1186/s12864-020-06860-z https://hdl.handle.net/10630/38829 10.1186/s12864-020-06860-z Background: Zebrafish is a popular model organism, which is widely used in developmental biology research. Despite its general use, the direct comparison of the zebrafish and human oocyte transcriptomes has not been well studied. It is significant to see if the similarity observed between the two organisms at the gene sequence level is also observed at the expression level in key cell types such as the oocyte. Results: We performed single-cell RNA-seq of the zebrafish oocyte and compared it with two studies that have performed single-cell RNA-seq of the human oocyte. We carried out a comparative analysis of genes expressed in the oocyte and genes highly expressed in the oocyte across the three studies. Overall, we found high consistency between the human studies and high concordance in expression for the orthologous genes in the two organisms. According to the Ensembl database, about 60% of the human protein coding genes are orthologous to the zebrafish genes. Our results showed that a higher percentage of the genes that are highly expressed in both organisms show orthology compared to the lower expressed genes. Systems biology analysis of the genes highly expressed in the three studies showed significant overlap of the enriched pathways and GO terms. Moreover, orthologous genes that are commonly overexpressed in both organisms were involved in biological mechanisms that are functionally essential to the oocyte. Conclusions: Orthologous genes are concurrently highly expressed in the oocytes of the two organisms and these genes belong to similar functional categories. Our results provide evidence that zebrafish could serve as a valid model organism to study the oocyte with direct implications in human. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Ovulos - Aspectos genéticos Ingeniería genética Comparative analysis of single-cell transcriptomics in human and zebrafish oocytes journal article