2026-05-28T00:20:35Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/390072026-02-03T11:33:17Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Lavado-Benito, Carla
author
Murillo, Jesús
author
Martínez Gil, Marta
author
Ramos-Rodríguez, Cayo Juan
author
Rodríguez-Moreno, Luis Gabriel
2025-06-17T08:52:55Z
2025-06-17T08:52:55Z
2024-02-05
Front. Plant Sci. 15:1347982
https://hdl.handle.net/10630/39007
10.3389/fpls.2024.1347982
GacS/GacA is a widely distributed two-component system playing an essential
role as a key global regulator, although its characterization in phytopathogenic
bacteria has been deeply biased, being intensively studied in pathogens of
herbaceous plants but barely investigated in pathogens of woody hosts. P.
savastanoi pv. savastanoi (Psv) is characterized by inducing tumours in the
stem and branches of olive trees. In this work, the model strain Psv NCPPB
3335 and a mutant derivative with a complete deletion of gene gacA were
subjected to RNA-Seq analyses in a minimum medium and a medium mimicking
in planta conditions, accompanied by RT-qPCR analyses of selected genes and
phenotypic assays. These experiments indicated that GacA participates in the
regulation of at least 2152 genes in strain NCPPB 3335, representing 37.9 % of the
annotated CDSs. GacA also controls the expression of diverse rsm genes, and
modulates diverse phenotypes, including motility and resistance to oxidative
stresses. As occurs with other P. syringae pathovars of herbaceous plants, GacA
regulates the expression of the type III secretion system and cognate effectors. In
addition, GacA also regulates the expression of WHOP genes, specifically
encoded in P. syringe strains isolated from woody hosts, and genes for the
biosynthesis of phytohormones. A gacA mutant of NCPPB 3335 showed
increased virulence, producing large immature tumours with high bacterial
populations, but showed a significantly reduced competitiveness in planta. Our
results further extend the role of the global regulator GacA in the virulence and
fitness of a P. syringae pathogen of woody hosts
eng
Attribution 4.0 Internacional
Pseudomonas syringae - Genética
Bacterias fitopatógenas
Bacterias gram negativas
GacA reduces virulence and increases competitiveness in planta in the tumorigenic olive pathogen Pseudomonas savastanoi pv. savastanoi
journal article
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