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oai:riuma.uma.es:10630/390172026-02-03T11:05:31Zcom_10630_2254col_10630_37953

Pintado Calvillo, Adrián Pérez-Martínez, Isabel Aragón Cortés, Isabel María Gutiérrez Barranquero, José Antonio De-Vicente-Moreno, Antonio Cazorla-López, Francisco Manuel Ramos-Rodríguez, Cayo Juan 2025-06-17T10:03:19Z 2025-06-17T10:03:19Z 2021-06-25 Pintado, A.; Pérez-Martínez, I.; Aragón, I.M.; Gutiérrez-Barranquero, J.A.; de Vicente, A.; Cazorla, F.M.; Ramos, C. The Rhizobacterium Pseudomonas alcaligenes AVO110 Induces the Expression of Biofilm-Related Genes in Response to Rosellinia necatrix Exudates. Microorganisms 2021, 9, 1388. https://doi.org/10.3390/microorganisms9071388 https://hdl.handle.net/10630/39017 10.3390/microorganisms9071388 The rhizobacterium Pseudomonas alcaligenes AVO110 exhibits antagonism toward the phytopathogenic fungus Rosellinia necatrix. This strain efficiently colonizes R. necatrix hyphae and is able to feed on their exudates. Here, we report the complete genome sequence of P. alcaligenes AVO110. The phylogeny of all available P. alcaligenes genomes separates environmental isolates, including AVO110, from those obtained from infected human blood and oyster tissues, which cluster together with Pseudomonas otitidis. Core and pan-genome analyses showed that P. alcaligenes strains encode highly heterogenic gene pools, with the AVO110 genome encoding the largest and most exclusive variable region (~1.6 Mb, 1795 genes). The AVO110 singletons include a wide repertoire of genes related to biofilm formation, several of which are transcriptionally modulated by R. necatrix exudates. One of these genes (cmpA) encodes a GGDEF/EAL domain protein specific to Pseudomonas spp. strains isolated primarily from the rhizosphere of diverse plants, but also from soil and water samples. We also show that CmpA has a role in biofilm formation and that the integrity of its EAL domain is involved in this function. This study contributes to a better understanding of the niche-specific adaptations and lifestyles of P. alcaligenes, including the mycophagous behavior of strain AVO110 eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 Internacional Bacterias gram negativas Hongos vegetales Micología The rhizobacterium Pseudomonas alcaligenes AVO110 induces the expression of biofilm-related genes in response to Rosellinia necatrix exudates journal article