2026-06-01T04:33:38Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/390352026-02-03T11:06:08Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Álvarez-Torres, Daniel author Moreno, Patricia author García-Rosado, Esther author Alonso-Sánchez, María del Carmen author Béjar-Alvarado, Julia author 2025-06-12 Mx genes display strong and quick induction in response to viral infections, which varies according to the viral virulence; furthermore, mx transcription is blocked by several viruses as part of their immune evasion strategies. Therefore, the level and time course of mx induction reflect virus–host interplay. This idea prompted the development of in vitro experimental systems consisting of cells stably expressing luciferase under the control of fish mx promoters. In this study, two RTG-2 cell lines, expressing luciferase under the control of Senegalese sole (Solea senegalensis) mx (ssmx) promoter, and under the control of gilthead sea bream (Sparus aurata) mx2 (saumx2) promoter, have been applied to study betanodavirus–host interaction. Both systems were inoculated with nervous necrosis virus, NNV, isolates belonging to different genotypes. The combination of both systems has been proved to be useful to detect all of them, although each isolate triggered a characteristic profile in each system. In addition, a protocol to estimate the titre of the RGNNV isolate in sea bass (Dicentrarchus labrax) brain has been established, and a clear dose-dependent antagonistic effect of this NNV isolate was recorded. Thus, both cell lines are useful tools to study betanodavirus–host interaction and, therefore, contribute to developing measures to fight viral infections in aquaculture. Álvarez‐Torres, D., Moreno, P., García‐Rosado, E., Alonso, M. C., & Béjar, J. (2025). Betanodavirus Quantification and <scp>IFN</scp> I‐Antagonism Detection Using Luciferase Reporter Systems Based on Fish mx Promoters. Journal of Fish Diseases. 0140-7775 https://hdl.handle.net/10630/39035 10.1111/jfd.70000 Peces - Enfermedades por virus Betanodavirus Quantification and IFN I-Antagonism Detection Using Luciferase Reporter Systems Based on Fish mx Promoters