2026-06-01T08:29:50Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/391932026-02-03T10:56:56Zcom_10630_2254col_10630_37953

Intestinal permeability and immune-inflammatory markers in patients with idiosyncratic drug-induced liver injury, drug induced steatosis and metabolic dysfunction–associated steatotic liver disease (MASLD) Di Zeo-Sánchez, Daniel Enrique Díaz-Alberola, irene Pinazo-Bandera, José M. García-Cortés, Miren Sanabria-Cabrera, Judith Adriana Robles-Díaz, María Mercedes Álvarez-Álvarez, Ismael Lucena, M. Isabel Andrade, Raúl J. Villanueva-Paz, Marina Stephens, Camilla Farmacología Toxicología Inmunología Background and Purpose Adverse immuno-inflammatory responses possibly influenced by bacterial compounds reaching the liver as a consequence of altered intestinal permeability appear to be crucial in the pathogenesis of drug-induced liver injury and steatotic liver diseases. This study aimed to assess intestinal permeability and immuno-inflammatory status in patients by measuring indirect biomarkers. Experimental Approach Circulating marker levels were measured in serum and plasma samples of 36 healthy controls, 32 patients with drug-induced liver injury, 14 with autoimmune hepatitis, 13 with viral hepatitis, 40 with metabolic dysfunction–associated steatotic liver disease (MASLD) and 16 with drug-induced steatosis. All patients with acute liver injury were identified (visit 1) and followed for >30 days (visit 2). Correlation analyses were performed to determine potential associations. Key Results Drug-induced liver injury, autoimmune hepatitis and viral hepatitis patients had higher levels of LBP, CD14, CD163, MCSF-1R (CSFR) and ICAM-1 and significantly lower levels of MAdCAM-1 and zonulin at detection of liver injury compared with healthy controls or the second visit. Drug-induced steatosis and MASLD patients had increased levels of S100A9, S100A12 and zonulin. MASLD patients with significant fibrosis (F2–F4) also had higher levels of CD163 and MCSF-1R. No difference was found between drug-induced steatosis and MASLD with no or low fibrosis. Conclusion and Implications Our results highlight similarities in macrophage activation, intestinal barrier dysfunction and translocation of bacterial products in liver injury of various aetiologies. A better understanding of the pathophysiological mechanisms may aid the development of targeted therapies for liver inflammation and fibrosis. 2025-07-01T11:46:05Z 2025-07-01T11:46:05Z 2025-07-01T11:46:05Z 2025 journal article Di Zeo-Sánchez, D. E., Díaz-Alberola, I., Pinazo-Bandera, J. M., García-Cortés, M., Sanabria-Cabrera, J., Robles-Díaz, M., A´ lvarez-A´ lvarez, I., Lucena, M. I., Andrade, R. J., Villanueva-Paz, M., & Stephens, C. (2025). Intestinal permeability and immune-inflammatory markers in patients with idiosyncratic drug-induced liver injury, drug-induced steatosis and metabolic dysfunction–associated steatotic liver disease (MASLD). British Journal of Pharmacology, 1–14. https://doi.org/10.1111/bph.70123 https://hdl.handle.net/10630/39193 10.1111/bph.70123 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley