2026-05-28T09:29:13Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/394982026-02-03T10:49:11Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Burgos-Molina, Antonio Manuel
author
Mercado-Sáenz, Silvia
author
Sendra-Portero, Francisco
author
Ruiz-Gómez, Miguel José
2025-07-25T08:53:33Z
2025-07-25T08:53:33Z
2020
Antonio M. Burgos-Molina, Silvia Mercado-Sáenz, Francisco Sendra- Portero & Miguel J. Ruiz-Gómez (2020) Effect of low frequency magnetic field on efficiency of chromosome break repair, Electromagnetic Biology and Medicine, 39:1, 30-37, DOI: 10.1080/15368378.2019.1685541
https://hdl.handle.net/10630/39498
10.1080/15368378.2019.1685541
DNA repair is essential to maintain genome integrity. There is scientific evidence that exposure to magnetic fields (MF) can produce alterations in DNA repair without clear conclusions. This work aims to study the cellular response to and repair of a very deleterious type of DNA damage, the DNA double strand break (DSB), in S. cerevisiae, under MF exposure. In S. cerevisiae cells, pairs of DSB were induced enzymatically by HO endonuclease by plating the cells on Galactose-containing media. The repair processes took place under exposure to a 50Hz, 2.45mT sinusoidal MF during 21 days. MF was generated by a pair of Helmholtz coils. MF induced 1.29- and 1.5-fold increase in the number of colonies grown at day 21 of exposure in relation to untreated controls for Pho91 and Rmd5 strain, respectively. In relation to the kinetics of DSB repair during MF exposure, a higher increase (55.56-fold) in DNA reparation was observed at day 15 for Rmd5 strain in relation to the slight increment (1.18-fold) found for Pho91 strain. The results suggest that long-term MF exposure could increase the DNA repair activity and there may be a relationship between the position of the DSB and the distance to the centromere.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Acido desoxirribonucleico - Reparación
Saccharomyces cerevisiae
Effect of low frequency magnetic field on efficiency of chromosome break repair
journal article
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URL
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c70ca758dffa7840fac3947e96462811
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application/pdf
LEBM-2019-0030.R1_- manuscript R1 -Aceptado.pdf