2026-05-27T23:27:37Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/397332026-02-03T11:24:25Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Martin‐Masot, Rafael author Correa-López, Nerea author Herrador-López, Marta author Navas-López, Víctor Manuel author Carmona, Francisco David author Nestares, Teresa author Bossini-Castillo, Lara author 2025-08-11 Celiac disease (CD) is an autoimmune disorder with a strong genetic component, triggered by gluten ingestion. Although a Gluten-Free Diet (GFD) is the standard treatment, its short-term effects on immune cell modulation in pediatric CD remain largely unexplored. This study aimed to investigate transcriptional changes in peripheral blood mononuclear cells (PBMCs) of pediatric CD patients following a strict GFD for 9–10 months, using single-cell RNA sequencing (scRNA-seq). An observational longitudinal study was conducted on five pediatric CD patients pre-GFD and post-GFD (confirmed by gluten immunogenic peptide determination in feces). PBMCs were analyzed using droplet-based scRNA-seq to identify cluster markers and differentially expressed genes (DEGs) between pre-GFD and post-GFD cells. Nineteen immune cell clusters encompassing a variety of classical immune cell subtypes were identified. Key findings included the downregulation of pro-inflammatory genes and the upregulation of immune-regulatory genes after a GFD in different immune cell subsets. Changes in macrophages and monocytes suggested improved immune balance, while T cells demonstrated a shift towards reduced effector activity. Notably, post-GFD regulatory T cells transitioned into a trajectory towards enhanced immunosuppressive profiles, as evidenced by increased HLA-G and decreased DDX5 expression. A strict short-term GFD induced significant immune modulation in pediatric CD patients, highlighting potential biomarkers for disease monitoring. Nevertheless, due to the small sample size, results should be interpreted with caution, and larger cohort studies are needed for further confirmation and validation. These findings provide insights into the immunological mechanisms of GFD and suggest avenues for non-invasive diagnostic strategies to enhance patient management. Martín-Masot R, Correa-López N, Herrador-López M, Navas-López VM, Carmona FD, Nestares T, Bossini-Castillo L. Immunomodulatory effects of a short-term gluten-free diet on pediatric celiac disease: findings from a single-cell transcriptomics study. J Nutr Biochem. 2025 Aug 11:110063. doi: 10.1016/j.jnutbio.2025.110063 https://hdl.handle.net/10630/39733 10.1016/j.jnutbio.2025.110063 Enfermedad celíaca en niños Secuenciación de ácidos nucleicos Respuesta inmune - Regulación Células T Pediatría Dietas sin gluten Immunomodulatory effects of a short-term gluten-free diet on pediatric celiac disease: findings from a single-cell transcriptomics study.