2026-05-31T06:21:20Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/399302026-02-03T11:11:46Zcom_10630_2254col_10630_37953

Alteration of IGF-1 bioavailability due to PAPPA2 deficiency leads to sex-specific metabolic disturbances López-Gambero, Antonio J. Vargas, Antonio Fernández-Arjona, María del Mar Rubio-Lamia, Leticia Olga De Ceglia, Marialuisa Vera-Fernández, Carlos Campillo-Calatayud, Ana Rivera-González, Patricia Rodriguez-de-Fonseca, Fernando Barrios, Vicente Chowen, Julie A. Argente, Jesús Suárez-Pérez, Juan Endocrinología Metabolismo - Aspectos endocrinos Hormonas Diferencias sexuales Homeostasis Background: The growth hormone (GH)/insulin-like growth factor (IGF-1) axis determines optimal growth and affects metabolism and energy homeostasis. Pregnancy-associated plasma protein-A2 (PAPPA2) regulates bioactive IGF-1 availability and patients with PAPPA2 deficiency have impaired growth and glucose metabolism. This axis is altered in metabolic disturbances such as obesity and anorexia nervosa in a sex-specific manner, but the mechanisms involved are not completely understood. Here we evaluated how Pappa2 deficiency affects energy homeostasis, focusing on male and female differences. Methods: Growth and energy homeostasis were determined in male and female Pappa2ko/ko mice and control Pappa2wt/wt littermates, as well as their response to recombinant human (rh)PAPPA2, rhIGF-1 and rhIBFBP5. Effects of a high-carbohydrate diet (HCHD) on glucose tolerance, fuel partitioning, de novo lipogenesis and energy homeostasis were determined. Results: Pappa2ko/ko mice had reduced body weight, bone length and lipid deposition associated with higher energy expenditure and intake. Male Pappa2ko/ko mice had mild glucose intolerance, altered bone mineral properties and higher energy costs for locomotor activity possibly due to inefficient muscle mitochondrial activity; whereas female Pappa2ko/ko mice had enhanced fatty acid oxidation on a normal diet, but not on a HCHD. All Pappa2ko/ko mice had lower hepatic fat deposition associated with lower IGF-1 activity in the liver, while fatty acid metabolism dysregulation in adipose tissue was found only in females. Conclusion: These data reinforce the importance of the GH/IGF-1 axis in metabolic control and emphasize the relevance of its fine-tuned control by Pappa2. Moreover, the differences between sexes in metabolic imbalances underscore the relevance of sex-specific strategies for treatment of metabolic imbalances. 2025-09-16T08:55:40Z 2025-09-16T08:55:40Z 2025-10 journal article Antonio J. López-Gambero, Antonio Vargas, María del Mar Fernández-Arjona, Leticia Rubio, Marialuisa de Ceglia, Carlos Vera-Fernández, Ana Campillo-Calatayud, Patricia Rivera, Fernando Rodríguez de Fonseca, Vicente Barrios, Julie A. Chowen, Jesús Argente, Juan Suárez, Alteration of IGF-1 bioavailability due to PAPPA2 deficiency leads to sex-specific metabolic disturbances, Metabolism, Volume 171, 2025, 156355, ISSN 0026-0495, https://doi.org/10.1016/j.metabol.2025.156355. (https://www.sciencedirect.com/science/article/pii/S0026049525002240) https://hdl.handle.net/10630/39930 10.1016/j.metabol.2025.156355 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional ELSEVIER