2026-05-29T22:31:47Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/401622026-02-03T11:11:39Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Vega-Granados, Karla author Escobar-Ibarra, Paola author Palomino-Vizcaino, Kenia author Cruz-Reyes, Juan author Valverde-Guillén, Piedad author Latorre-Redoli, Sofía author Caneda-Santiago, Candela T. author Mari-Beffa, Manuel author Romero-Sánchez, Lilian B. author 2024-06-25 Ammonium and hexyltrimethylammonium thiomolybdates (ATM and ATM-C6) and thiotungstates (ATT and ATT-C6) were synthesized. Their toxicity was evaluated using both in vitro and in vivo approaches via the zebrafish embryo acute toxicity assay (ZFET), while the copper-thiometallate interaction was studied using cyclic voltammetry, as well as in an in vivo assay. Cyclic voltammetry suggests that all thiometallates form complexes with copper in a 2:1 Cu:thiometallate ratio. Both in vitro and in vivo assays demonstrated low toxicity in BALB/ 3T3 cells and in zebrafish embryos, with high IC50 and LC 50 values. Furthermore, the hexyltrimethylammonium ion played a crucial role in enhancing viability and reducing toxicity during prolonged treatments for ATM and ATT. In particular, the ZEFT assay uncovered the accumulation of ATM in zebrafish yolk, averted by the incorporation of the hexyltrimethylammonium ion. Notably, the copper-thiometallate interaction assay high lighted the improved viability of embryos when cultured in CuCl2 and ATM-C6, even at high CuCl2 concentrations. The hatching assay further confirmed that copper-ATM-C6 interaction mitigates inhibitory effects induced by thiomolybdates and CuCl2 when administered individually. These results suggest that the incorporation of the hexyltrimethylammonium ion in ATM increase its ability to interact with copper and its potential application as a copper chelator. Vega-Granados, K., Escobar-Ibarra, P., Palomino-Vizcaino, K., Cruz-Reyes, J., Valverde-Guillén, P., Latorre-Redoli, S., Caneda-Santiago, C. T., Marí-Beffa, M., & Romero-Sánchez, L. B. (2024). Hexyltrimethylammonium ion enhances potential copper-chelating properties of ammonium thiomolybdate in an in vivo zebrafish model. Archives of biochemistry and biophysics, 758, 110077. https://doi.org/10.1016/j.abb.2024.110077 https://hdl.handle.net/10630/40162 10.1016/j.abb.2024.110077 Peces cebra Voltametría Análisis electroquímico Quelatoterapia Cobre Hexyltrimethylammonium ion enhances potential copper-chelating properties of ammonium thiomolybdate in an in vivo zebrafish model.