2026-05-27T05:23:14Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/401682026-02-03T11:29:47Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Pliego, Clara author Crespo-Gómez, José Ignacio author Pintado Calvillo, Adrián author Pérez-Martínez, Isabel author De Vicente, Antonio author Cazorla-López, Francisco Manuel author Ramos-Rodríguez, Cayo Juan author 2019-01-23 The rhizobacterium Pseudomonas pseudoalcaligenes AVO110, isolated by the enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused by Rosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudatecontaining medium with the goal of identifying the molecular mechanisms linked to the interaction of this bacterium with R. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and named growth-attenuated mutants (GAMs). Twenty-one genes were identified as being required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase (recB) and a regulator of alginate production (algQ) were identified as being required for efficient colonization of the avocado rhizosphere. Pliego C, Crespo-Gómez JI, Pintado A, Pérez-Martínez I, de Vicente A, Cazorla FM, Ramos C. 2019. Response of the biocontrol agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix exudate. Appl Environ Microbiol 85:e01741-18. https://doi.org/10 .1128/AEM.01741-18 https://hdl.handle.net/10630/40168 10.1128/AEM.01741-18 Agentes de control biológico de plagas Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate