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oai:riuma.uma.es:10630/401682026-02-03T11:29:47Zcom_10630_2254col_10630_37953

Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate Pliego, Clara Crespo-Gómez, José Ignacio Pintado Calvillo, Adrián Pérez-Martínez, Isabel De Vicente, Antonio Cazorla-López, Francisco Manuel Ramos-Rodríguez, Cayo Juan Agentes de control biológico de plagas Biocontrol Pseudomonas pseudoalcaligenes Rosellinia necatrix Exudate The rhizobacterium Pseudomonas pseudoalcaligenes AVO110, isolated by the enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused by Rosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudatecontaining medium with the goal of identifying the molecular mechanisms linked to the interaction of this bacterium with R. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and named growth-attenuated mutants (GAMs). Twenty-one genes were identified as being required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase (recB) and a regulator of alginate production (algQ) were identified as being required for efficient colonization of the avocado rhizosphere. 2025-10-10T09:23:08Z 2025-10-10T09:23:08Z 2019-01-23 journal article VoR Pliego C, Crespo-Gómez JI, Pintado A, Pérez-Martínez I, de Vicente A, Cazorla FM, Ramos C. 2019. Response of the biocontrol agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix exudate. Appl Environ Microbiol 85:e01741-18. https://doi.org/10 .1128/AEM.01741-18 https://hdl.handle.net/10630/40168 10.1128/AEM.01741-18 eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf American Society fo Microbilogy