2026-05-30T04:36:24Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/403642026-02-03T11:28:45Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Suárez-Pérez, Juan author Rivera-González, Patricia author Arrabal, Sergio author Crespillo, Ana author Serrano, Antonia author Baixeras-Llano, Elena author Pavón, Francisco Javier author Cifuentes-Rueda, Manuel author Nogueiras, Ruben author Ballesteros, Joan author Diéguez, Carlos author Rodríguez de Fonseca, Fernando author 2014-01 β-adrenergic receptor activation promotes brown adipose tissue (BAT) β-oxidation and thermogenesis by burning fatty acids during uncoupling respiration. Oleoylethanolamide (OEA) can inhibit feeding and stimulate lipolysis by activating peroxisome proliferator-activating receptor-α (PPARα) in white adipose tissue (WAT). Here we explore whether PPARα activation potentiates the effect of β3-adrenergic stimulation on energy balance mediated by the respective agonists OEA and CL316243. The effect of this pharmacological association on feeding, thermogenesis, β-oxidation, and lipid and cholesterol metabolism in epididymal (e)WAT was monitored. CL316243 (1 mg/kg) and OEA (5 mg/kg) co-administration over 6 days enhanced the reduction of both food intake and body weight gain, increased the energy expenditure and reduced the respiratory quotient (VCO2/VO2). This negative energy balance agreed with decreased fat mass and increased BAT weight and temperature, as well as with lowered plasma levels of triglycerides, cholesterol, nonessential fatty acids (NEFAs), and the adipokines leptin and TNF-α. Regarding eWAT, CL316243 and OEA treatment elevated levels of the thermogenic factors PPARα and UCP1, reduced p38-MAPK phosphorylation, and promoted brown-like features in the white adipocytes: the mitochondrial (Cox4i1, Cox4i2) and BAT (Fgf21, Prdm16) genes were overexpressed in eWAT. The enhancement of the fatty-acid β-oxidation factors Cpt1b and Acox1 in eWAT was accompanied by an upregulation of de novo lipogenesis and reduced expression of the unsaturated-fatty-acid-synthesis enzyme gene, Scd1. We propose that the combination of β-adrenergic and PPARα receptor agonists promotes therapeutic adipocyte remodelling in eWAT, and therefore has a potential clinical utility in the treatment of obesity. Juan Suárez*, Patricia Rivera*, Sergio Arrabal, Ana Crespillo, Antonia Serrano, Elena Baixeras, Francisco J Pavón, Manuel Cifuentes, Rubén Nogueiras, Joan Ballesteros, Carlos Dieguez, Fernando Rodríguez de Fonseca. Oleoylethanolamide enhances β-adrenergic-mediated thermogenesis and white-to-brown adipocyte phenotype in epididymal white adipose tissue in rat. Dis Model Mech. 2014 Jan;7(1):129-41. doi: 10.1242/dmm.013110. https://hdl.handle.net/10630/40364 10.1242/dmm.013110 Fisología Factores de transcripción Proteínas Adrenalina - Receptores Células adiposas Oleoylethanolamide enhances β-adrenergic-mediated thermogenesis and white-to-brown adipocyte phenotype in epididymal white adipose tissue. in rat