2026-05-27T20:40:01Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/404572026-02-03T11:28:56Zcom_10630_2254col_10630_37953

Toral, Laura Rodríguez-González, Miguel Ángel Martínez-Checa, Fernando Montaño, Alfredo Cortés-Delgado, Amparo Smolinska, Agnieszka Llamas, Inmaculada Sampedro, Inmaculada 2025-10-24T12:14:45Z 2025-10-24T12:14:45Z 2021-11-12 Toral L, Rodríguez M, Martínez-Checa F, Montaño A, Cortés-Delgado A, Smolinska A, Llamas I and Sampedro I (2021) Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens. Front. Microbiol. 12:773092. doi: 10.3389/fmicb.2021.773092 https://hdl.handle.net/10630/40457 10.3389/fmicb.2021.773092 10.3389/fmicb.2023.1267324 Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/ MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Compuestos orgánicos volátiles Hongos fitopatógenos Agentes antifúngicos Control biológico Frutas - Almacenamiento - Enfermedades y daños Hortalizas - Almacenamiento - Enfermedades y daños Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens journal article